SP(Substance P) ELISA Kit
中文名称:P物质(SP)酶联免疫吸附测定试剂盒
别称:
TAC1、TAC2、NKNA
Price:
Size:
96T
48T
96T*5
- 反应性: Universal
- 检测范围: 78.13-5000 pg/mL
- 灵敏度: 46.88 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用SP抗原包被于酶标板上,实验时样品或标准品中的SP与包被的SP竞争生物素标记的抗SP单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,SP浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中SP的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Universal |
检测方法 | Colormetric |
检测范围 | 78.13-5000 pg/mL |
灵敏度 | 46.88 pg/mL |
样本体积 | 50μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的SP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
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Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by Ligilactobacillus salivarius Li01
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A randomised, double-blind, placebo-controlled trial of Bifidobacterium bifidum CCFM16 for manipulation of gut microbiota and relief from chronic constipation
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Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa
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NO up-regulates migraine-related CGRP via activation of an Akt/GSK-3β/NF-κB signaling cascade in trigeminal ganglion neurons
IF: 4.831Journal:Aging-US -
Protective effect of the combination of essential oil from patchouli and tangerine peel against gastric ulcer in rats
IF: 4.36 -
Atractylodes oil alleviates diarrhea-predominant irritable bowel syndrome by regulating intestinal inflammation and intestinal barrier via SCF/c-kit and MLCK/MLC2 pathways
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Laxative effect and mechanism of Tiantian Capsule on loperamide-induced constipation in rats
IF: 4.36 -
Acute Effect of Transcutaneous Electroacupuncture on Globus Pharyngeus: A Randomized, Single-Blind, Crossover Trial
IF: 3.9 -
Cervical sympathectomy modulates the neurogenic inflammatory neuropeptides following experimental subarachnoid hemorrhage in rats
IF: 2.929