Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit
中文名称:总谷胱甘肽(T-GSH)/氧化型谷胱甘肽(GSSG)比色法测试盒
Price:
Size:
100Assays(96 samples)
50Assays(48 samples)
- 样本类型: 血清(浆);动物组织;红细胞;细胞
- 检测仪器及波长: 紫外-可见光分光光度计(412 nm)
- 研究领域: 氧化应激
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
通过谷胱甘肽还原酶把GSSG还原成GSH,而GSH可以和生色底物DTNB反应产生GSSG和黄色的TNB,总谷胱甘肽(GSSG+GSH)的量决定了黄色的TNB形成量。从而通过测定A412(波长在412 nm处时的吸光度值)就可以计算出总谷胱甘肽的量。再用适当试剂先清除样品中的GSH然后利用上述反应原理就可以测定出GSSG的含量。
|
灵敏度 | 0.12 μmol/L T-GSH |
检测范围 | 0.12-30 μmol/L T-GSH |
精密度 | 批间差:4.7 | 批内差:0.9 |
检测时间 | 180 min |
检测仪器及波长 | 紫外-可见光分光光度计(412 nm) |
保存条件 | 该产品可在 2~8°C/-20°C避光条件下保存6个月,具体试剂组分的保存条件请参考说明书。 |
有效期 | 6个月 |
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