EPI(Epinephrine/Adrenaline) ELISA Kit
中文名称:肾上腺素(EPI)酶联免疫吸附测定试剂盒
别称:
Epinephrine、Adrenaline、Adrenalin、4,5-β-trihydroxy-N-methylphenethylamine
Price:
Size:
96T
48T
96T*5
- 反应性: Universal
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用EPI抗原包被于酶标板上,实验时样品或标准品中的EPI与包被的EPI竞争生物素标记的抗EPI单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,EPI浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中EPI的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Universal |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 50μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的EPI,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Light modulates glucose metabolism by a retina-hypothalamus-brown adipose tissue axis
IF: 66.85 -
β-adrenoreceptor-triggered PKA activation negatively regulates the innate antiviral response
IF: 22.096 -
Neuroendocrine stress hormones associated with short-term exposure to nitrogen dioxide and fine particulate matter in individuals with and without chronic obstructive pulmonary disease: A panel study in Beijing, China
IF: 9.988 -
Uncoupling protein -1 modulates anxiety-like behavior in a temperature-dependent manner
IF: 6.709 -
Maternal anxiety affects embryo implantation via impairing adrenergic receptor signaling in decidual cells
IF: 6.548 -
Metformin prevents the pathological browning of subcutaneous white adipose tissue
IF: 6.181Journal:Molecular Metabolism -
Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors
IF: 6.018Journal:Computational and Structural Biotechnology JournalPMID:33304464 -
Caffeine supplementation induces higher IL-6 and IL-10 plasma levels in response to a treadmill exercise test
IF: 5.068Journal:Journal of the International Society of Sports NutritionPMID:32907591 -
Investigating the molecular mechanisms of delirium-like neuropsychiatric disorder induced by electromagnetic pulse based on bioinformatics analysis
IF: 4.399Journal:Molecular Brain -
Deep transcranial magnetic stimulation in combination with skin thermography in obesity: a window on sympathetic nervous system
IF: 4.28