EPI(Epinephrine/Adrenaline) ELISA Kit
中文名称:肾上腺素(EPI)酶联免疫吸附测定试剂盒
别称:
Epinephrine、Adrenaline、Adrenalin、4,5-β-trihydroxy-N-methylphenethylamine
Price:
Size:
96T
48T
96T*5
- 反应性: Universal
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用EPI抗原包被于酶标板上,实验时样品或标准品中的EPI与包被的EPI竞争生物素标记的抗EPI单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,EPI浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中EPI的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Universal |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 50μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的EPI,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Beneficial Effects of lncRNA-UC.360+ shRNA on Diabetic Cardiac Sympathetic Damage via NLRP3 Inflammasome-Induced Pyroptosis in Stellate Ganglion
IF: 4.132 -
Protective effect of low-dose radiation on doxorubicin-induced brain injury in mice
IF: 4.114 -
A Case Control Study on Serum Levels of Potential Biomarkers in Male Breast Cancer Patients
IF: 3.39Journal:International Journal of Environmental Research and Public HealthPMID:34062830 -
Perivascular radiofrequency renal denervation lowers blood pressure and ameliorates cardiorenal fibrosis in spontaneously hypertensive rats
IF: 3.24Journal:PLoS One -
Intranasal Dexmedetomidine on Stress Hormones, Inflammatory Markers, and Postoperative Analgesia after Functional Endoscopic Sinus Surgery
IF: 3.236Journal:MEDIATORS OF INFLAMMATION -
High frequency deep transcranial magnetic stimulation acutely increases β-endorphins in obese humans
IF: 3.179Journal:ENDOCRINE -
Selective ablation of the ligament of Marshall reduces ischemia and reperfusion-induced ventricular arrhythmias
IF: 2.766 -
A Novel Swine Model of Spontaneous Hypertension With Sympathetic Hyperactivity Responds Well to Renal Denervation
IF: 2.689Journal:AMERICAN JOURNAL OF HYPERTENSION -
LianXia Formula Granule Attenuates Cardiac Sympathetic Remodeling in Rats with Myocardial Infarction via the NGF/TrKA/PI3K/AKT Signaling Pathway
IF: 2.63Journal:Evidence-based Complementary and Alternative MedicinePMID:34221073 -
Primary stress response and biochemical profile of Labeo rohita (Hamilton, 1822) experimentally parasitized with Argulus bengalensis (Ramakrishna, 1951)
IF: 2.051