Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)

货号：E-AB-1011
文献（10）
说明书

说明书

Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)

Price: ¥840.00 ¥210.00 ¥135.00 ¥80.00 ¥1470.00

Size:

500μL 120μL 60μL 30μL 1mL

宿主： Goat
应用性： IF

Source	Goat
Immunogen	Mouse IgG
Application	IF
Recommended Dilution	IF 1:50-100
Concentration	0.5 mg/mL(specific antibody purification≥99%),CY3:Ab=3-9:1
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	Cyanine 3
Amax	554nm
Emax	568-574nm
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Storage Buffer	PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4

Accelerative effect of nanohydrogels based on chitosan/ZnO incorporated with citral to heal the infected full-thickness wounds; an experimental study

IF: 8.025

Journal：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

DOI：10.1016/j.ijbiomac.2022.07.038

PMID：35820486
Fabrication of novel polysaccharide hybrid nanoliposomes containing citral for targeting MRSA-infected wound healing

IF: 6.76

Journal：JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY

DOI：10.1016/j.jiec.2022.11.004
Nanoliposomal peptides derived from Spirulina platensis protein accelerate full-thickness wound healing

IF: 6.51

Journal：INTERNATIONAL JOURNAL OF PHARMACEUTICS

DOI：10.1016/j.ijpharm.2022.122457
A novel multifaceted approach for infected wound healing: Optimization and in vivo evaluation of Phenethyl alcohol loaded nanoliposomes hydrogel

IF: 5.062

Journal：JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY

DOI：10.1016/j.jddst.2022.103888
Biofabrication of ZnO/Malachite nanocomposite and its coating with chitosan to heal infectious wounds

IF: 4.996

Journal：Scientific Reports

DOI：10.1038/s41598-022-15768-5

PMID：35803975
Nerolidol inhibits the LOX-1 / IL-1β signaling to protect against the Aspergillus fumigatus keratitis inflammation damage to the cornea

IF: 3.943

Journal：INTERNATIONAL IMMUNOPHARMACOLOGY

DOI：10.1016/j.intimp.2019.106118

PMID：31926445
Astragaloside IV inhibits pathological functions of gastric cancer-associated fibroblasts

IF: 3.365

Journal：WORLD JOURNAL OF GASTROENTEROLOGY

DOI：10.3748/wjg.v23.i48.8512
Development of fluorescent lateral flow test strips based on an electrospun molecularly imprinted membrane for detection of triazophos residues in tap water

IF: 3.288

Journal：NEW JOURNAL OF CHEMISTRY

DOI：10.1039/D0NJ00269K
IL-17A Promotes the Migration, Invasion and the EMT Process of Lung Cancer Accompanied by NLRP3 Activation

IF: 3.246

Journal：Biomed Research International

DOI：10.1155/2022/7841279

PMID：36349316
Effectiveness of topical administration of platelet-rich plasma on the healing of methicillin-resistant Staphylococcus aureus infected full-thickness wound model

IF: 3.022

Journal：Journal of Plastic Reconstructive and Aesthetic Surgery

DOI：10.1016/j.bjps.2022.11.059

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。 ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色； WB主要选择HRP标记的二抗，用ECL底物发光液显色； IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色； IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。