Goat Anti-Mouse IgG(H+L)(FITC conjugated)

货号：E-AB-1015
文献（11）
说明书

说明书

Goat Anti-Mouse IgG(H+L)(FITC conjugated)

Price: ¥840.00 ¥210.00 ¥135.00 ¥80.00 ¥1470.00

Size:

500μL 120μL 60μL 30μL 1mL

宿主： Goat
应用性： IF

Source	Goat
Immunogen	Mouse IgG
Application	IF
Recommended Dilution	IF 1:20-100
Concentration	0.5 mg/mL(specific antibody purification≥99%),FITC:Ab=4-6:1
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	FITC
Amax	495nm
Emax	525nm
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Storage Buffer	PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4

Multiple Tolerization Subtractive Immunization (MTSI) Protocol: Effects on Mice and Monoclonal Antibody Specificity.

IF: 7.561

Journal：Frontiers in Immunology

DOI：10.3389/fimmu.2021.760817
Secretory phosphoprotein 1 secreted by fibroblast-like synoviocytes promotes osteoclasts formation via PI3K/AKT signaling in collagen-induced arthritis

IF: 7.419

Journal：BIOMEDICINE & PHARMACOTHERAPY

DOI：10.1016/j.biopha.2022.113687

PMID：36088855
PSTPIP2 regulates synovial macrophages polarization and dynamics via ERβ in the joint microenvironment

IF: 5.606

Journal：ARTHRITIS RESEARCH & THERAPY

DOI：10.1186/s13075-022-02939-y
Hyperuricemia induces lipid disturbances mediated by LPCAT3 upregulation in the liver

IF: 4.966

Journal：FASEB JOURNAL

DOI：10.1096/fj.202000950R

PMID：32780898
Rewritable magnetic fluorescence-encoded microspheres: preparation, characterization, and recycling

IF: 4.696

Journal：Journal of Materials Chemistry C

DOI：10.1039/C5TC01301A
Functional HLA-C expressing trophoblast spheroids as a model to study placental–maternal immune interactions during human implantation

IF: 4.38

Journal：Scientific Reports

DOI：10.1038/s41598-022-12870-6
Visfatin protein may be responsible for suppression of proliferation and apoptosis in the infantile mice ovary

IF: 3.861

Journal：CYTOKINE

DOI：10.1016/j.cyto.2021.155422

PMID：33476980
Inhibition of visfatin/NAMPT affects ovarian proliferation, apoptosis, and steroidogenesis in pre-pubertal mice ovary

IF: 3.813

Journal：JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

DOI：10.1016/j.jsbmb.2020.105763

PMID：32987128
The Role of IL-6 and TMEM100 in Lumbar Discogenic Pain and the Mechanism of the Glycine-Serine-Threonine Metabolic Axis: A Metabolomic and Molecular Biology Study

IF: 2.832

Journal：Journal of Pain Research

DOI：10.2147/JPR.S400871
Changes in the localization of ovarian visfatin protein and its possible role during estrous cycle of mice

IF: 2.107

Journal：ACTA HISTOCHEMICA

DOI：10.1016/j.acthis.2020.151630

PMID：32992122

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。 ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色； WB主要选择HRP标记的二抗，用ECL底物发光液显色； IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色； IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。