Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 594 conjugated)

货号：E-AB-1060
文献（8）
说明书

说明书

Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 594 conjugated)

Price: ¥840.00 ¥210.00 ¥135.00 ¥80.00 ¥1470.00

Size:

500μL 120μL 60μL 30μL 1mL

宿主： Goat
应用性： IF

Source	Goat
Immunogen	Rabbit IgG
Application	IF
Recommended Dilution	IF 1:100-300
Concentration	0.5 mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	Elab Fluor®594
Amax	591nm
Emax	614nm
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Storage Buffer	PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4

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PMID：36063961
Biodegradable mesoporous nanocomposites with dual-targeting function for enhanced anti-tumor therapy

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RNF114 Silencing Inhibits the Proliferation and Metastasis of Gastric Cancer

IF: 4.207

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Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis

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Journal：LIFE SCIENCES

DOI：10.1016/j.lfs.2020.118118

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。 ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色； WB主要选择HRP标记的二抗，用ECL底物发光液显色； IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色； IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。