Goat Anti-Rabbit IgG(H+L)(FITC conjugated)

货号：E-AB-1014
文献（17）
说明书

说明书

Goat Anti-Rabbit IgG(H+L)(FITC conjugated)

Price: ¥840.00 ¥210.00 ¥135.00 ¥80.00 ¥1470.00

Size:

500μL 120μL 60μL 30μL 1mL

宿主： Goat
应用性： IF

Source	Goat
Immunogen	Rabbit IgG
Application	IF
Recommended Dilution	IF 1:20-100
Concentration	0.5 mg/mL(specific antibody purification≥99%),FITC:Ab=4-6:1
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	FITC
Amax	495nm
Emax	525nm
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Storage Buffer	PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4

Accelerative effect of nanohydrogels based on chitosan/ZnO incorporated with citral to heal the infected full-thickness wounds; an experimental study

IF: 8.025

Journal：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

DOI：10.1016/j.ijbiomac.2022.07.038

PMID：35820486
Fabrication of novel polysaccharide hybrid nanoliposomes containing citral for targeting MRSA-infected wound healing

IF: 6.76

Journal：JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY

DOI：10.1016/j.jiec.2022.11.004
Nanoliposomal peptides derived from Spirulina platensis protein accelerate full-thickness wound healing

IF: 6.51

Journal：INTERNATIONAL JOURNAL OF PHARMACEUTICS

DOI：10.1016/j.ijpharm.2022.122457
Caspase-8 is involved in pyroptosis, necroptosis and the maturation and release of IL-1β in Aspergillus fumigatus keratitis

IF: 5.714

Journal：INTERNATIONAL IMMUNOPHARMACOLOGY

DOI：10.1016/j.intimp.2022.109275

PMID：36274488
A novel multifaceted approach for infected wound healing: Optimization and in vivo evaluation of Phenethyl alcohol loaded nanoliposomes hydrogel

IF: 5.062

Journal：JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY

DOI：10.1016/j.jddst.2022.103888
Fabrication, characterization and application of novel nanoemulsion polyvinyl alcohol/chitosan hybrid incorporated with citral for healing of infected full-thickness wound

IF: 5.062

Journal：JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY

DOI：10.1016/j.jddst.2022.103589
Effects of polybrene and retronectin as transduction enhancers on the development and phenotypic characteristics of VHH-based CD19-redirected CAR T cells: a comparative investigation

IF: 5.057

Journal：CLINICAL AND EXPERIMENTAL MEDICINE

DOI：10.1007/s10238-022-00928-8
Carnosic acid protects against ferroptosis in PC12 cells exposed to erastin through activation of Nrf2 pathway

IF: 5.037

Journal：LIFE SCIENCES

DOI：10.1016/j.lfs.2020.118905

PMID：33333051
Ameliorative effects of echinacoside against spinal cord injury via inhibiting NLRP3 inflammasome signaling pathway

IF: 5.037

Journal：LIFE SCIENCES

DOI：10.1016/j.lfs.2019.116978

PMID：31644893
Biofabrication of ZnO/Malachite nanocomposite and its coating with chitosan to heal infectious wounds

IF: 4.996

Journal：Scientific Reports

DOI：10.1038/s41598-022-15768-5

PMID：35803975

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。 ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色； WB主要选择HRP标记的二抗，用ECL底物发光液显色； IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色； IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。