Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)

货号：E-AB-1003
文献（224）
说明书

说明书

Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)

Price: ¥640.00 ¥160.00 ¥105.00 ¥70.00 ¥1200.00

Size:

500 μL 120 μL 60 μL 30 μL 1 mL

宿主： Goat
应用性： WB;IHC;ELISA

Source	Goat
Immunogen	Rabbit IgG
Application	WB, IHC, ELISA
Recommended Dilution	WB 1:5000-50000(Enhanced chemiluminescent detection) WB 1:1000-5000(DAB detection) Direct ELISA 1:5000-30000(TMB detection) IHC 1:500-2000(DAB detection)
Concentration	0.5 mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	HRP
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Storage Buffer	PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4

Ultrasmall Coordination Polymer Nanodots Fe-Quer Nanozymes for Preventing and Delaying the Development and Progression of Diabetic Retinopathy

IF: 19.000

Journal： ADVANCED FUNCTIONAL MATERIALS (2023)

DOI：10.1002/adfm.202300261

Reactivity：Rat

Sample Type：retinal tissue
Smart Telomerase-Gated DNA cage for precise siRNA release in cancer cells

IF: 15.100

Journal： CHEMICAL ENGINEERING JOURNAL (2023)

DOI：10.1016/j.cej.2023.143782

Reactivity：Human
Transdermal Sustained Release Properties and Anti-Photoaging Efficacy of Liposome-Thermosensitive Hydrogel System

IF: 10.000

Journal： Advanced Healthcare Materials (2023)

DOI：10.1002/adhm.202301933

PMID：37607774

Reactivity：Guinea pig

Sample Type：skin
Constructing epidermal rete ridges using a composite hydrogel to enhance multiple signaling pathways for the maintenance of epidermal stem cell niche

IF: 9.700

Journal： Acta Biomaterialia (2023)

DOI：10.1016/j.actbio.2023.07.037

PMID：37516415

Reactivity：Human
Study of anti-fatigue activity of polysaccharide from fruiting bodies of Armillaria gallica

IF: 8.200

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2023)

DOI：10.1016/j.ijbiomac.2023.124611

PMID：37119895

Reactivity：Mouse

Sample Type：muscle,liver tissues
Radix Astragali decoction improves liver regeneration by upregulating hepatic expression of aquaporin-9

IF: 7.900

Journal： PHYTOMEDICINE (2023)

DOI：10.1016/j.phymed.2023.155166

PMID：37918281

Reactivity：Mouse

Sample Type：liver
Inflammatory stimulation of astrocytes affects the expression of miRNA-22-3p within NSCs-EVs regulating remyelination by targeting KDM3A

IF: 7.500

Journal： Stem Cell Research & Therapy (2023)

DOI：10.1186/s13287-023-03284-w

PMID：36959678

Reactivity：Rat

Sample Type：spinal cord
A new approach for the treatment of bleomycin-induced rat pulmonary injury by combined protein fraction of major royal jelly protein 2 and its isoform X1

IF: 7.500

Journal： BIOMEDICINE & PHARMACOTHERAPY (2023)

DOI：10.1016/j.biopha.2023.115578

PMID：37742609

Reactivity：Rat

Sample Type：lung tissue
Identification of a novel immune-related gene signature for prognosis and the tumor microenvironment in patients with uveal melanoma combining single-cell and bulk sequencing data

IF: 7.300

Journal： Frontiers in Immunology (2023)

DOI：10.3389/fimmu.2023.1099071

PMID：36793711

Reactivity：Human
Anti-Inflammatory and Anti-Oxidative Effects of Polysaccharides Extracted from Unripe Carica papaya L. Fruit

IF: 7.000

Journal： Antioxidants (2023)

DOI：10.3390/antiox12081506

PMID：37627501

Reactivity：Human

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。 ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色； WB主要选择HRP标记的二抗，用ECL底物发光液显色； IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色； IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。