Human AD(Adropin) ELISA Kit
中文名称:人Adropin蛋白(AD)酶联免疫吸附测定试剂盒
别称:
ENHO、Energy Homeostasis Associated
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 12.5-800 pg/mL
- 灵敏度: 7.5 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人AD抗体包被于酶标板上,实验时样品(或标准品)中的人AD会与包被抗体结合。后依次加入生物素化的抗人AD抗体和辣根过氧化物酶标记的亲和素,抗人AD抗体与结合在包被抗体上的人AD结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,AD浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中AD的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法 |
检测范围 | 12.5-800 pg/mL |
灵敏度 | 7.5 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人AD,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
The Impact of SGLT2 Inhibitor Dapagliflozin on Adropin Serum Levels in Men and Women with Type 2 Diabetes Mellitus and Chronic Heart Failure
IF: 4.757Journal:Biomedicines -
Interplay between Myokine Profile and Glycemic Control in Type 2 Diabetes Mellitus Patients with Heart Failure
IF: 3.992Journal:Diagnostics -
Association between adropin and coronary artery lesions in children with Kawasaki disease
IF: 3.183 -
A novel biomarker in patients with knee osteoarthritis: adropin
IF: 2.141Journal:CLINICAL RHEUMATOLOGY -
Serum irisin and adropin levels may be predictors for coronary artery ectasia
IF: 1.749 -
Alterations in Serum Adropin, Adiponectin, and Proinflammatory Cytokine Levels in OSAS
IF: 1.639 -
Association between serum adropin levels and isolated coronary artery ectasia in patients with stable angina pectoris
IF: 1.112Journal:Anatolian Journal of Cardiology -
Serum Adropin Levels Are Reduced in Adult Patients with Nonalcoholic Fatty Liver Disease
IF: 1.103Journal:MEDICAL PRINCIPLES AND PRACTICE -
Plasma Adropin as a Potential Marker Predicting Obesity and Obesity-associated Cancer in Korean Patients With Type 2 Diabetes Mellitus