Human BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
中文名称:人脑源性神经营养因子(BDNF)酶联免疫吸附测定试剂盒
别称:
ANON2、BULN2
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人BDNF抗体包被于酶标板上,实验时样品(或标准品)中的人BDNF会与包被抗体结合。后依次加入生物素化的抗人BDNF抗体和辣根过氧化物酶标记的亲和素,抗人BDNF抗体与结合在包被抗体上的人BDNF结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,BDNF浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中BDNF的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人BDNF,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
LncRNA ANRIL mediates endothelial dysfunction through BDNF downregulation in chronic kidney disease
IF: 9.685 -
Insulin-like growth factor-1 attenuates oxidative stress-induced hepatocyte premature senescence in liver fibrogenesis via regulating nuclear p53–progerin interaction
IF: 5.959Journal:Cell Death & Disease -
Sublingual buprenorphine/naloxone treatment is not affected by OPRM1 A118G and BDNF Va66Met polymorphisms, but alters the plasma beta-endorphin and BDNF levels in individuals with opioid use disorder
IF: 5.785 -
Effects of 12 Weeks Cosmos caudatus Supplement among Older Adults with Mild Cognitive Impairment: A Randomized, Double-Blind and Placebo-Controlled Trial
IF: 5.719 -
A randomised, double-blind, placebo-controlled trial of Bifidobacterium bifidum CCFM16 for manipulation of gut microbiota and relief from chronic constipation
IF: 5.396Journal:Food & Function -
Effects of erinacine A-enriched Hericium erinaceus on elderly hearing-impaired patients: A double-blind, randomized, placebo-controlled clinical trial
IF: 5.223Journal:Journal of Functional Foods -
The Effects of Four Weeks of Chiropractic Spinal Adjustments on Blood Biomarkers in Adults with Chronic Stroke: Secondary Outcomes of a Randomized Controlled Trial
IF: 4.964Journal:Journal of Clinical Medicine -
Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression
IF: 4.791 -
The role of BDNF and NGF plasma levels in first-episode schizophrenia: A longitudinal study
IF: 4.6 -
Adult human periodontal ligament-derived stem cells delay retinal degeneration and maintain retinal function in RCS rats
IF: 4.211Journal:Stem Cell Research & Therapy