Human BMP-2(Bone Morphogenetic Protein 2) ELISA Kit
中文名称:人骨成型蛋白2(BMP-2)酶联免疫吸附测定试剂盒
别称:
BMP2A、BMP-2A、BDA2
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 62.5-4000 pg/mL
- 灵敏度: 37.5 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人BMP-2抗体包被于酶标板上,实验时样品(或标准品)中的人BMP-2会与包被抗体结合。后依次加入生物素化的抗人BMP-2抗体和辣根过氧化物酶标记的亲和素,抗人BMP-2抗体与结合在包被抗体上的人BMP-2结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,BMP-2浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中BMP-2的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 62.5-4000 pg/mL |
灵敏度 | 37.5 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人BMP-2,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Regulation of macrophage subtype via injectable micro/nano-structured porous microsphere for reprogramming osteoimmune microenvironment
IF: 13.273Journal:CHEMICAL ENGINEERING JOURNAL -
Role of molecular and chemical chaperon in assisting refolding of BMP2 in E. coli
IF: 8.025Journal:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULESPMID:35970369 -
Early Osteogenic Marker Expression in hMSCs Cultured onto Acid Etching-Derived Micro- and Nanotopography 3D-Printed Titanium Surfaces
IF: 6.208 -
Comparative effects of virtual reality training and sensory motor training on bone morphogenic proteins and inflammatory biomarkers in post-traumatic osteoarthritis
IF: 3.998 -
Role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction
IF: 2.634 -
Experimental study of rhBMP-2 chitosan nano-sustained release carrier-loaded PLGA/nHA scaffolds to construct mandibular tissue-engineered bone
IF: 1.663 -
Prognostic Value of Bone Formation and Resorption Proteins in Heterotopic Ossification in Critically-Ill Patients. a Single-Centre Study.
Journal:Journal of Critical Care Medicine