Human Cyt-C(Cytochrome C) ELISA Kit
中文名称:人细胞色素C(Cyt-C)酶联免疫吸附测定试剂盒
别称:
CytC、CYCS、CYC、HCS、THC4
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 78.13-5000 pg/mL
- 灵敏度: 46.88 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人Cyt-C抗体包被于酶标板上,实验时样品(或标准品)中的人Cyt-C会与包被抗体结合。后依次加入生物素化的抗人Cyt-C抗体和辣根过氧化物酶标记的亲和素,抗人Cyt-C抗体与结合在包被抗体上的人Cyt-C结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Cyt-C浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中Cyt-C的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 78.13-5000 pg/mL |
灵敏度 | 46.88 pg/mL |
样本体积 | 100μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人Cyt-C,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Altered expression of ERK, Cytochrome-c, and HSP70 triggers apoptosis in Quinacrine-exposed human invasive ductal carcinoma cells
IF: 6.53 -
The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes
IF: 5.784Journal:FREE RADICAL BIOLOGY AND MEDICINE -
Response of Circulating Inflammatory Markers to Intermittent Hypoxia-Hyperoxia Training in Healthy Elderly People and Patients with Mild Cognitive Impairment
IF: 3.817Journal:Life-Basel -
Chronic inflammation is a key to inducing liver injury in blunt snout bream (Megalobrama amblycephala) fed with high-fat diet
IF: 3.119 -
Mitochondrial DNA-Induced Inflammatory Responses and Lung Injury in Thermal Injury Murine Model: Protective Effect of Cyclosporine-A
IF: 1.538 -
Data in the activities of caspases and the levels of reactive oxygen species and cytochrome c in the •OH-induced fish erythrocytes treated with alanine, citrulline, proline and their combination
Journal:Data in Brief -
Effect of senescence on some apoptosis and oxidative stress markers in infertile normozospermic and oligospermic men: A cross-sectional study
Journal:International Journal of Reproductive BiomedicinePMID:30234183
Q1:Cytochrome-C, Cytochrome P450 aromatase What is the difference between these two indicators?
Cytochrome-c: the most abundant and stable Cytochrome. Participate in energy transfer; Cytochrome P450 aromatase: A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androgen-4-ene-3, 17-dione (androstenedione) and testosterone to C18 estrogens, estrone and estradiol, respectively.