Human FGF23(Fibroblast Growth Factor 23) ELISA Kit
中文名称:人成纤维细胞生长因子23(FGF23)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人FGF23抗体包被于酶标板上,实验时样品(或标准品)中的人FGF23会与包被抗体结合。后依次加入生物素化的抗人FGF23抗体和辣根过氧化物酶标记的亲和素,抗人FGF23抗体与结合在包被抗体上的人FGF23结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,FGF23浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中FGF23的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人FGF23,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss
IF: 7.08 -
Kernelized k-Local Hyperplane Distance Nearest-Neighbor Model for Predicting Cerebrovascular Disease in Patients With End-Stage Renal Disease
IF: 4.677 -
Body composition, adipokines, FGF23-Klotho and bone in kidney transplantation: Is there a link?
IF: 3.902 -
Correlation study of FGF23/D-serine in maintenance hemodialysis patients with combined hearing impairment
IF: 3.752Journal:PLoS One -
The Association of Fibroblast Growth Factor 23 with Arterial Stiffness and Atherosclerosis in Patients with Autosomal Dominant Polycystic Kidney Disease
IF: 3Journal:KIDNEY & BLOOD PRESSURE RESEARCH -
The correlation between fibroblast growth factor-23 and ESRD patients with hearing impairment
IF: 2.984 -
Fibroblast growth factor 23 in children with or without heart failure: a prospective study
IF: 2.926Journal:BMJ Paediatrics Open -
Association of plasma neutrophil gelatinase-associated lipocalin with parameters of CKD–MBD in maintenance hemodialysis patients
IF: 2.626Journal:JOURNAL OF BONE AND MINERAL METABOLISM -
Association Between FGF-23 Levels and Risk of Fracture in Women With Systemic Sclerosis
IF: 2.31 -
A Self-Representation-Based Fuzzy SVM Model for Predicting Vascular Calcification of Hemodialysis Patients
IF: 2.238