Human GDF15(Growth Differentiation Factor 15) ELISA Kit
中文名称:人生长分化因子15(GDF15)酶联免疫吸附测定试剂盒
别称:
GDF-15、MIC-1、MIC1、NAG-1、PDF、PLAB、PTGFB、TGF-PL
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 23.44-1500 pg/mL
- 灵敏度: 14.06 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人GDF15抗体包被于酶标板上,实验时样品(或标准品)中的人GDF15会与包被抗体结合。后依次加入生物素化的抗人GDF15抗体和辣根过氧化物酶标记的亲和素,抗人GDF15抗体与结合在包被抗体上的人GDF15结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,GDF15浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中GDF15的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 23.44-1500 pg/mL |
灵敏度 | 14.06 pg/mL |
样本体积 | 100μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人GDF15,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Assessment of serum tenascin-C and growth differentiation factor-15 among type 2 diabetes mellitus patients with and without acute coronary syndrome
IF: 3.402 -
Evaluation of cardiovascular risk by growth-differentiation factor-15 and tissue Doppler imaging in children with subclinical hypothyroidism
IF: 3.296Journal:ENDOCRINE -
Comparison of the Cytokine Profile in Mesenchymal Stem Cells from Human Adipose, Umbilical Cord, and Placental Tissues
IF: 1.987 -
Growth Differentiation Factor-15 Level and Tissue Doppler Echocardiography as a Tool in Identification of Cardiac Effects in the Children with Type 1 Diabetes Mellitus
IF: 1.927 -
Additional Diagnostic Value of Growth Differentiation Factor-15 (GDF-15) to N-Terminal B-Type Natriuretic Peptide (NT-proBNP) in Patients with Different Stages of Heart Failure
IF: 1.894Journal:MEDICAL SCIENCE MONITOR -
Increased serum concentrations of growth differentiation factor-15 in children with acute rheumatic fever
IF: 1.093