Human IL-6(Interleukin 6) ELISA Kit
中文名称:人白介素6(IL-6)酶联免疫吸附测定试剂盒
别称:
IL6、BSF2、HGF、HSF、IFNB2、CDF
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 1.56-100 pg/mL
- 灵敏度: 0.94 pg/mL
用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上,实验时样品或标准品中的人IL-6会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-6浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-6的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 1.56-100 pg/mL |
灵敏度 | 0.94 pg/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人IL-6,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1
IF: 27.401 -
Increased Liver Localization of Lipopolysaccharides in Human and Experimental NAFLD
IF: 17.425 -
Overcoming the Heat Endurance of Tumor Cells by Interfering with the Anaerobic Glycolysis Metabolism for Improved Photothermal Therapy
IF: 13.942Journal:ACS Nano -
Double-Layer Nanofibrous Sponge Tube via Electrospun Fiber and Yarn for Promoting Urethral Regeneration
IF: 12.958Journal:Advanced Fiber Materials -
A SCID mouse-human lung xenograft model of SARS-CoV-2 infection
IF: 11.556 -
Combined effect of polystyrene microplastics and bisphenol A on the human embryonic stem cells-derived liver organoids: The hepatotoxicity and lipid accumulation
IF: 10.753 -
Biodegradable MoSe2-polyvinylpyrrolidone nanoparticles with multi-enzyme activity for ameliorating acute pancreatitis
IF: 10.435 -
High mobility group box 1 derived mainly from platelet microparticles exacerbates microvascular obstruction in no reflow
IF: 10.407Journal:THROMBOSIS RESEARCH -
Light-Mediated 3D Printing of Micro-Pyramid-Decorated Tailorable Wound Dressings with Endogenous Growth Factor Sequestration for Improved Wound Healing
IF: 10.383Journal:ACS Applied Materials & Interfaces -
Developmental exposure to chlorpyrifos causes neuroinflammation via necroptosis in mouse hippocampus and human microglial cell line
IF: 9.988
E-UNEL-H0097 | Uncoated Human IL-6(Interleukin 6) ELISA Kit | 96T*5 , 96T*15 |