Human NES1(Nesfatin 1) ELISA Kit
中文名称:人Nesfatin 1蛋白(NES1)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人NES1抗体包被于酶标板上,实验时样品(或标准品)中的人NES1会与包被抗体结合。后依次加入生物素化的抗人NES1抗体和辣根过氧化物酶标记的亲和素,抗人NES1抗体与结合在包被抗体上的人NES1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,NES1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中NES1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人NES1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Evaluating the impact of metabolic and cognitive stress on ghrelin and nesfatin-1 hormones in patients with diabetes and diabetic depression
IF: 3.000 -
Associations between Oxidant/Antioxidant Status and Circulating Adipokines in Non-Obese Children with Prader–Willi Syndrome
IF: 7.000 -
Circulating Levels of Nesfatin-1 and Spexin in Children with Prader-Willi Syndrome during Growth Hormone Treatment and Dietary Intervention
IF: 5.900 -
Analysis of Interleukin-17, Interleukin-23, neopterin and Nesfatin-1 levels in the sera of Hashimoto patients
IF: 2.500 -
Association between nesfatin-1 hormone levels, anthropometric measurements, and glucose regulation shortly after sleeve gastrectomy: A?cross-sectional study
IF: 0.900Journal: International Journal of Diabetes in Developing Countries (2023)Reactivity:HumanSample Type:serum -
Altered levels of neurobiological biomarkers at the interface of depression and gestational diabetes mellitus in Asian Indian women
IF: 3.152 -
Serum nesfatin-1, ghrelin, and lipid levels in adolescents with first episode drug na?ve unipolar depression
IF: 1.780 -
Is there any association between low level of serum nesfatin-1 and fibromyalgia syndrome?
-
The association of low levels of nesfatin-1 and glucagon-like peptide-1 with oxidative stress in Parkinson’s disease
IF: 2.484 -
The association of serum nesfatin-1 and ghrelin levels with metabolic syndrome in patients with schizophrenia
IF: 2.528