Human S100A9(S100 Calcium Binding Protein A9) ELISA Kit
中文名称:人S100钙结合蛋白A9(S100A9)酶联免疫吸附测定试剂盒
别称:
MRP14、6B8AG、CAGB、CFAG、CGLB、L1AG、LIAG、MAC387、MIF、NIF、P14、Calgranulin-B
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.31-20 pg/mL
- 灵敏度: 0.12 pg/mL
用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中S100A9浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人S100A9抗体包被于酶标板上,实验时样品(或标准品)中的人S100A9会与包被抗体结合。后依次加入生物素化的抗人S100A9抗体和辣根过氧化物酶标记的亲和素,抗人S100A9抗体与结合在包被抗体上的人S100A9结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,S100A9浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中S100A9的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法 |
检测范围 | 0.31-20 pg/mL |
灵敏度 | 0.12 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人S100A9,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
IL-17 stimulates neutrophils to release S100A8/A9 to promote lung epithelial cell apoptosis in Mycoplasma pneumoniae–induced pneumonia in children
IF: 6.53 -
Translational Proteomic Approach for Cholangiocarcinoma Biomarker Discovery, Validation, and Multiplex Assay Development: A Pilot Study
IF: 4.927 -
TLR4 and RAGE conversely mediate pro-inflammatory S100A8/9-mediated inhibition of proliferation-linked signaling in myeloproliferative neoplasms
IF: 4.761Journal:CELLULAR ONCOLOGY -
Obesity, Metabolic syndrome, Diabetes
IF: 3.432Journal:CLINICAL CHEMISTRY AND LABORATORY MEDICINE -
Proteomics investigation of OSCC-specific salivary biomarkers in a Hungarian population highlights the importance of identification of population-tailored biomarkers
IF: 3.24Journal:PLoS One -
Tacrolimus Inhibits TNF-α/IL-17A-Produced pro-Inflammatory Effect on Human Keratinocytes by Regulating IκBζ
IF: 2.939 -
Enhanced S100A9 and S100A12 expression in acute coronary syndrome
IF: 2.02Journal:Biomarkers in Medicine -
Macrophage mannose receptor 1 and S100A9 were identified as serum diagnostic biomarkers for colorectal cancer through a label-free quantitative proteomic analysis
IF: 1.736Journal:Cancer Biomarkers