Human SEMA3A(Semaphorin 3A) ELISA Kit
中文名称:人信号素3A(SEMA3A)酶联免疫吸附测定试剂盒
别称:
SEMA3A、COLL1、HH16、Hsema-I、Hsema-III、SEMA1、SEMAD、SEMAIII、SEMAL、SemD、Coll-1、Semaphorin 3A
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人SEMA3A抗体包被于酶标板上,实验时样品(或标准品)中的人SEMA3A会与包被抗体结合。后依次加入生物素化的抗人SEMA3A抗体和辣根过氧化物酶标记的亲和素,抗人SEMA3A抗体与结合在包被抗体上的人SEMA3A结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,SEMA3A浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中SEMA3A的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人SEMA3A,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Osteoblasts are inherently programmed to repel sensory innervation
IF: 11.508Journal:Bone Research -
Axon guidance molecule semaphorin3A is a novel tumor suppressor in head and neck squamous cell carcinoma
IF: 5.008Journal:Oncotarget -
Gingival crevicular fluid lipocalin-2 and semaphorin3A in stage III periodontitis: Non-surgical periodontal treatment effects
IF: 4.419 -
Assessment of semaphorin 3A and semaphorin 7A levels in primary Sjogren's syndrome
IF: 3.58 -
Extract of Scutellaria baicalensis induces semaphorin 3A production in human epidermal keratinocytes
IF: 3.24 -
SEMA3A suspended in matrigel improves titanium implant fixation in ovariectomized rats
IF: 2.881Journal:JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS -
Identification and Functional Characterization of a Novel Variant in the SEMA3A Gene in a Chinese Family with Kallmann Syndrome
IF: 2.803Journal:International Journal of Endocrinology -
Decreased expression of Sema3A, an immune modulator, in blood sample of multiple sclerosis patients
IF: 2.415Journal:GENE -
Evaluation of serum semaphorin 3A and interleukin 6 levels in patients with pseudoexfoliation syndrome
IF: 2.029Journal:INTERNATIONAL OPHTHALMOLOGY -
The Neuropilin‐1 Ligand, Sema3A, Acts as a Tumor Suppressor in the Pathogenesis of Acute Leukemia
IF: 1.373Journal:Anatomical Record-Advances in Integrative Anatomy and Evolutionary BiologyDOI:10.1002/ar.24016