Mouse BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
中文名称:小鼠脑源性神经营养因子(BDNF)酶联免疫吸附测定试剂盒
别称:
ANON2、BULN2
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠BDNF抗体包被于酶标板上,实验时样品(或标准品)中的小鼠BDNF会与包被抗体结合。后依次加入生物素化的抗小鼠BDNF抗体和辣根过氧化物酶标记的亲和素,抗小鼠BDNF抗体与结合在包被抗体上的小鼠BDNF结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,BDNF浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中BDNF的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠BDNF,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Bifidobacterium breve intervention combined with environmental enrichment alleviates cognitive impairment by regulating the gut microbiota and microbial metabolites in Alzheimer's disease mice.
IF: 8.786Journal:Frontiers in Immunology -
Systemic Beta-Hydroxybutyrate Affects BDNF and Autophagy into the Retina of Diabetic Mice
IF: 6.208 -
BDNF Augmentation Using Riluzole Reverses Doxorubicin-Induced Decline in Cognitive Function and Neurogenesis
IF: 6.088Journal:Neurotherapeutics -
Anti-Epileptic Effect of Crocin on Experimental Temporal Lobe Epilepsy in Mice
IF: 5.811Journal:Frontiers in Pharmacology -
Administration of Bifidobacterium breve Improves the Brain Function of Aβ1-42-Treated Mice via the Modulation of the Gut Microbiome
IF: 5.719 -
Kynurenine monooxygenase inhibition and associated reduced quinolinic acid reverses depression-like behaviour by upregulating Nrf2/ARE pathway in mouse model of depression: In-vivo and In-silico studies
IF: 5.251 -
Clerodendrum viscosum leaves attenuate lead-induced neurotoxicity through upregulation of BDNF-Akt-Nrf2 pathway in mice
IF: 5.195Journal:JOURNAL OF ETHNOPHARMACOLOGY -
Memory-enhancing effects of 7,3′,4′-trihydroxyisoflavone by regulation of cholinergic function and BDNF signaling pathway in mice
IF: 4.679Journal:FOOD AND CHEMICAL TOXICOLOGY -
Fluoxetine ameliorates depressive symptoms by regulating lncRNA expression in the mouse hippocampus
IF: 4.56 -
Antidepressant-like effect of sodium orthovanadate in a mouse model of chronic unpredictable mild stress
IF: 4.432