Mouse HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
中文名称:小鼠低氧诱导因子1α(HIF-1α)酶联免疫吸附测定试剂盒
别称:
HIF1A、HIF-1A、MOP1、PASD8、BHLHe78、Basic helix-loop-helix transcription factor
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 125-8000 pg/mL
- 灵敏度: 75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠HIF-1α抗体包被于酶标板上,实验时样品(或标准品)中的小鼠HIF-1α会与包被抗体结合。后依次加入生物素化的抗小鼠HIF-1α抗体和辣根过氧化物酶标记的亲和素,抗小鼠HIF-1α抗体与结合在包被抗体上的小鼠HIF-1α结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,HIF-1α浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中HIF-1α的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 125-8000 pg/mL |
灵敏度 | 75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠HIF-1α,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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