Mouse HMGB-1(Highmobility group protein B1) ELISA Kit
中文名称:小鼠高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠HMGB-1抗体包被于酶标板上,实验时样品(或标准品)中的小鼠HMGB-1会与包被抗体结合。后依次加入生物素化的抗小鼠HMGB-1抗体和辣根过氧化物酶标记的亲和素,抗小鼠HMGB-1抗体与结合在包被抗体上的小鼠HMGB-1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,HMGB-1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中HMGB-1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠HMGB-1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Galectin-3 Deficiency Facilitates TNF-α-Dependent Hepatocyte Death and Liver Inflammation in MCMV Infection
IF: 4.259 -
miR-129-5p alleviates LPS-induced acute kidney injury via targeting HMGB1/TLRs/NF-kappaB pathway
IF: 3.943 -
Deficiency of PKCλ/ι alleviates the liver pathologic impairment of Schistosoma japonicum infection by thwarting Th2 response
IF: 3.876 -
JAK2/STAT1-mediated HMGB1 translocation increases inflammation and cell death in a ventilator-induced lung injury model
IF: 3.684 -
Hydrogen Alleviates Neuronal Injury and Neuroinflammation Induced by Microglial Activation via the Nuclear Factor Erythroid 2-related Factor 2 Pathway in Sepsis-associated Encephalopathy
IF: 3.59 -
Re-exposure to alarmin HMGB1 and cytokine IL-1 beta induces differential innate immune response generation in mouse brain
IF: 3.478 -
Honokiol Reduces Fungal Load, Toll-Like Receptor-2, and Inflammatory Cytokines in Aspergillus fumigatus Keratitis
IF: 3.47 -
High-Mobility Group Box 1 Disrupts Metabolic Function with Cigarette Smoke Exposure in a Ceramide-Dependent Manner
IF: 3.226Journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES -
SIRT1-mediated HMGB1 deacetylation suppresses sepsis-associated acute kidney injury
IF: 3.164Journal:AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGYPMID:30379096 -
MiR-181-5p protects mice from sepsis via repressing HMGB1 in an experimental model
IF: 3.024Journal:European Review for Medical and Pharmacological SciencesPMID:33015817