Mouse IL-1β(Interleukin 1 Beta) ELISA Kit

中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒

货号：E-EL-M0037
文献（437）
说明书

说明书

别称：

IL1B、IL1-BETA、IL1F2、Catabolin

Price: ¥2400 ¥1650 ¥10200

Size:

96T 48T 96T*5

反应性： Mouse
检测范围： 7.81-500 pg/mL
灵敏度： 4.69 pg/mL

产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。
反应类型	Sandwich-ELISA
规格	96T / 48T / 96T*5
反应时间	3.5h
反应性	Mouse
检测方法	Colormetric
检测范围	7.81-500 pg/mL
灵敏度	4.69 pg/mL
样本体积	100μL
样本类型	血清，血浆，细胞上清，细胞提取液体，组织匀浆等其他生物体液
特异性	可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃/-20℃
操作步骤
数据处理	ELISA结果计算

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Q1:Does the Mouse IL1B elisa test for precusor or mature IL1B?

The interleukin (IL)-1 family of cytokines is associated with immune response and inflammation. It consists of the following 11 different molecular forms: Intercellular IL-1α (IL-1F1), extracellular IL-1β (IL-1F2), IL-1 receptor antagonist (IL-1Ra, IL-1F3), And IL-18 (IL-1F4), IL-33 (IL-1F11), IL-36α (IL-1F6), IL-36β (IL-1F8), IL-36γ (IL-1F9), IL-36Ra (IL-1F5), IL-37 (IL-1F7), and IL-38 (L-1F10). One of the most important pro-inflammatory cytokines is IL-1β. It is primarily an inactive 31 kDa precursor produced by monocytes and macrophages, interleukin-1β (proIL-1β), which under the action of cysteine protease IL-1 convertase (ICE) produces a mature and biologically active 17.5kDa protein IL-1β.The E-EL-M0037 kit detects mature IL-1β.