Mouse PPAR-γ(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
中文名称:小鼠过氧化物酶体增殖因子活化受体γ(PPAR-γ)酶联免疫吸附测定试剂盒
别称:
glitazone receptor、NR1C3、PPARG、CIMT1、GLM1、PPARG1、PPARG2、PPARgamma、Nuclear receptor subfamily 1 group C member 3
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠PPAR-γ抗体包被于酶标板上,实验时样品(或标准品)中的小鼠PPAR-γ会与包被抗体结合。后依次加入生物素化的抗小鼠PPAR-γ抗体和辣根过氧化物酶标记的亲和素,抗小鼠PPAR-γ抗体与结合在包被抗体上的小鼠PPAR-γ结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,PPAR-γ浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中PPAR-γ的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠PPAR-γ,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Black corn (Zea mays L.) whole flour improved the antioxidant capacity and prevented the adipogenesis in mice fed a high-fat diet.
IF: 5.396Journal:Food & Function -
4-Thiazolidinone-based derivatives do not affect differentiation of mouse embryo fibroblasts (3T3-L1 cell line) into adipocytes
IF: 5.194 -
Calcium channel antagonists interfere with the mechanism of action of elastin-derived peptide VGVAPG in mouse cortical astrocytes in vitro
IF: 4.297 -
Artichoke (Cynara Scolymus) Methanolic Leaf Extract Alleviates Diethylnitrosamine-Induced Toxicity in BALB/c Mouse Brain: Involvement of Oxidative Stress and Apoptotically Related Klotho/PPARγ Signaling
IF: 3.508Journal:Journal of Personalized Medicine -
Triclosan affects the expression of nitric oxide synthases (NOSs), peroxisome proliferator-activated receptor gamma (PPARγ), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in mouse neocortical neurons in vitro
IF: 3.5 -
The Elastin-Derived Peptide VGVAPG Does Not Activate the Inflammatory Process in Mouse Cortical Astrocytes In Vitro
IF: 3.311Journal:NEUROTOXICITY RESEARCH -
Impact of elastin-derived VGVAPG peptide on bidirectional interaction between peroxisome proliferator-activated receptor gamma (Pparγ) and beta-galactosidase (β-Gal) expression in mouse cortical astrocytes in vitro
IF: 2.238Journal:NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY -
The effect of homocysteine on the expression of CD36, PPARγ, and C/EBPα in adipose tissue of normal and obese mice
IF: 2.11Journal:ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY -
Digested protein from chia seed (Salvia hispanica L) prevents obesity and associated inflammation of adipose tissue in mice fed a high-fat diet
Journal:PharmaNutrition