Mouse sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
中文名称：小鼠可溶性末端补体复合物sC5b-9(sC5b-9)酶联免疫吸附测定试剂盒
MAC、Membrane Attack Complex、C5b9、TCC C5b-9
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠sC5b-9抗体包被于酶标板上，实验时样品（或标准品）中的小鼠sC5b-9会与包被抗体结合。后依次加入生物素化的抗小鼠sC5b-9抗体和辣根过氧化物酶标记的亲和素，抗小鼠sC5b-9抗体与结合在包被抗体上的小鼠sC5b-9结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，sC5b-9浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中sC5b-9的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96T
/ 48T
/ 96T*5
|
| 反应时间 | 3.5h |
| 反应性 | Mouse |
| 检测方法 | 比色法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100μL |
| 样本类型 | 血清，血浆，细胞上清，细胞提取液体，组织匀浆等其他生物体液 |
| 特异性 | 可检测样本中的小鼠sC5b-9，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃/-20℃ |
| 数据处理 |
Q1:Does the sC5b-9(sC5b-9) kit detect activated or unactivated MAC, because MAC is present on the cell, so where is the main source of dissolved MAC? Is this result representative of MAC formation on cells?
The sC5b-9 kit detects the total, both active and inactive forms. MAC is present on cells, but when the multifunctional glycoprotein S protein (vitronectin) binds to the liquid phase of C5b9, it forms a complex that cannot attach to cells, called SC5b9. SC5b-9 is a membrane-attacking complex present in plasma. At abnormally elevated concentrations, SC5B-9 exhibits cytotoxicity, interacts with cytoskeletal components or membrane proteins to produce non-lethal effects on cells, and plays an important role in the pathological process of certain diseases. This kit measures the concentration of sC5b-9, which is generally used to study disease models related to sC5b-9 indicators, and can show changes in concentration between groups, but cannot represent the formation process of MAC on cells.
