Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
中文名称:小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上,实验时样品(或标准品)中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素,抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TNF-α浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TNF-α的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠TNF-α,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Adipose-Derived Stem Cells-Derived Exosomes with High Amounts of Circ_0001747 Alleviate Hypoxia/Reoxygenation-Induced Injury in Myocardial Cells by Targeting MiR-199b-3p/MCL1 Axis
IF: 1.862 -
Antioxidant and Anti-Inflammatory Activities of Kigelia africana (Lam.) Benth.
IF: 1.813Journal:Evidence-based Complementary and Alternative Medicine -
Curcumin affects ox‑LDL‑induced IL‑6, TNF‑α, MCP‑1 secretion and cholesterol efflux in THP‑1 cells by suppressing the TLR4/NF‑κB/miR33a signaling pathway
IF: 1.785Journal:Experimental and Therapeutic Medicine -
Impact of Lesinurad and allopurinol on experimental Hyperuricemia in mice: biochemical, molecular and Immunohistochemical study
IF: 1.771 -
Bone marrow mesenchymal stem cells accelerate the hyperglycemic refractory wound healing by inhibiting an excessive inflammatory response
IF: 1.692Journal:Molecular Medicine Reports -
The association between inflammation, the microbiome and urethane-induced pulmonary adenocarcinoma Corrigendum in /10.3892/ol.2018.9063
IF: 1.664Journal:Oncology Letters -
Chemerin-9 Peptide Enhances Memory and Ameliorates Aβ1-42-induced Object Memory Impairment in Mice
IF: 1.54Journal:BIOLOGICAL & PHARMACEUTICAL BULLETIN -
Aerofloral investigation and allergenic potentials of two dominant airborne pollen types at selected sites in South-western Nigeria
IF: 1.515Journal:AEROBIOLOGIA -
NLRP3 inflammasome signaling as an early molecular response is negatively controlled by miR-186 in CFA-induced prosopalgia mice
IF: 1.492Journal:BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH -
Preadipocytes In Vitro
IF: 1.484Journal:AESTHETIC PLASTIC SURGERY