Mouse TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit
中文名称:小鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶联免疫吸附测定试剂盒
别称:
ACP5、TRAP5b、SPENCDI
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 0.78-50 ng/mL
- 灵敏度: 0.47 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠TRACP-5b抗体包被于酶标板上,实验时样品(或标准品)中的小鼠TRACP-5b会与包被抗体结合。后依次加入生物素化的抗小鼠TRACP-5b抗体和辣根过氧化物酶标记的亲和素,抗小鼠TRACP-5b抗体与结合在包被抗体上的小鼠TRACP-5b结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TRACP-5b浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TRACP-5b的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 0.78-50 ng/mL |
灵敏度 | 0.47 ng/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠TRACP-5b,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Smart Nanosacrificial Layer on the Bone Surface Prevents Osteoporosis through Acid–Base Neutralization Regulated Biocascade Effects
IF: 14.612 -
Sirt3-mediated mitophagy regulates AGEs-induced BMSCs senescence and senile osteoporosis
IF: 11.799 -
Genesis of osteoclasts on calcium phosphate ceramics and their role in material-induced bone formation
IF: 10.633Journal:Acta Biomaterialia -
Dietary nitrate improves jaw bone remodelling in zoledronate-treated mice
IF: 8.755Journal:CELL PROLIFERATION -
Thiaplakortone B attenuates RANKL‐induced NF‐κB and MAPK signaling and dampens OVX‐induced bone loss in mice
IF: 7.419Journal:BIOMEDICINE & PHARMACOTHERAPY -
Aromatic Bis[aminomethylidenebis(phosphonic)] Acids Prevent Ovariectomy-Induced Bone Loss and Suppress Osteoclastogenesis in Mice
IF: 5.924 -
Brief Report: Consecutive Alendronate Administration‐Mediated Inhibition of Osteoclasts Improves Long‐Term Engraftment Potential and Stress Resistance of HSCs
IF: 5.902Journal:STEM CELLS -
Hymenialdisine: A Marine Natural Product That Acts on Both Osteoblasts and Osteoclasts and Prevents Estrogen-Dependent Bone Loss in Mice
IF: 5.854Journal:JOURNAL OF BONE AND MINERAL RESEARCH -
N,N-Dimethylformamide inhibits high glucose-induced osteoporosis via attenuating MAPK and NF-κB signalling
IF: 5.853Journal:Bone & Joint Research -
Dectin-1 signaling inhibits osteoclastogenesis via IL-33-induced inhibition of NFATc1
IF: 5.168Journal:Oncotarget