MS Mouse IGF-1(Insulin-like Growth Factor 1) ELISA Kit
中文名称:MS-小鼠胰岛素样生长因子1(IGF-1)酶联免疫吸附测定试剂盒
别称:
IGF1、IGFI、IGF-I、IGF1A、IGF-IA、IGF-IB、MGF、Somatomedin C
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 1.25-80 ng/mL
- 灵敏度: 0.75 ng/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IGF-1浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IGF-1抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IGF-1会与包被抗体结合。后依次加入生物素化的抗小鼠IGF-1抗体和辣根过氧化物酶标记的亲和素,抗小鼠IGF-1抗体与结合在包被抗体上的小鼠IGF-1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IGF-1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IGF-1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 1.25-80 ng/mL |
灵敏度 | 0.75 ng/mL |
样本体积 | 25μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠IGF-1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Retinol-Binding Protein 4 Accelerates Metastatic Spread and Increases Impairment of Blood Flow in Mouse Mammary Gland Tumors
IF: 6.126Journal:Cancers -
Maternal omega-3 fatty acid deficiency affects fetal thermogenic development and postnatal musculoskeletal growth in mice
IF: 6.117Journal:JOURNAL OF NUTRITIONAL BIOCHEMISTRY -
Emodin and rhein decrease levels of hypoxia-inducible factor-1α in human pancreatic cancer cells and attenuate cancer cachexia in athymic mice carrying these cells
IF: 5.168Journal:Oncotarget -
The Warburg effect in human pancreatic cancer cells triggers cachexia in athymic mice carrying the cancer cells
IF: 3.288Journal:BMC CANCER -
The effect of maternal obesity on the expression and functionality of placental P-glycoprotein: Implications in the individualized transplacental digoxin treatment for fetal heart failure
IF: 2.71Journal:PLACENTA -
Serum Proteins as New Biomarkers for Whole-Body Exposure to High- and Low-LET Ionizing Radiation:
IF: 2.438Journal:Dose-Response -
Antihypernociceptive, Anxiolytic, and Antidepressant Properties of Aqueous and Ethanol Extracts of Dissotis thollonii Cogn. (Melastomataceae) in Mice
Journal:Advances in Pharmacological and Pharmaceutical SciencesPMID:33015629
E-EL-M3006 | Mouse IGF-1(Insulin-like Growth Factor 1) ELISA Kit | 96T , 48T , 96T*5 |