MS Mouse IL-1α(Interleukin 1Alpha) ELISA Kit
中文名称：MS-小鼠白介素1α(IL-1α)酶联免疫吸附测定试剂盒
别称：
IL1A、IL1-A、IL1、IL1F1、Preinterleukin 1 Alpha、Hematopoietin-1、Pro-Interleukin-1-Alpha
Price:
Size:
96T
48T
96T*5
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 3.01 pg/mL
| 系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4，能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术，旨在帮助您以更高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-1α浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1α会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1α抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1α抗体与结合在包被抗体上的小鼠IL-1α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96T
/ 48T
/ 96T*5
|
| 反应时间 | 3.5h |
| 反应性 | Mouse |
| 检测方法 | Colormetric |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 3.01 pg/mL |
| 样本体积 | 25μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃/-20℃ |
| 数据处理 |
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The elevated level of IL-1α in the bone marrow of aged mice leads to MSC senescence partly by down-regulating Bmi-1
IF: 4.032 -
10-Hydroxydecanoic acid inhibits LPS-induced inflammation by targeting p53 in microglial cells
IF: 3.943Journal：INTERNATIONAL IMMUNOPHARMACOLOGY
| E-EL-M3059 | Mouse IL-1α(Interleukin 1 Alpha) ELISA Kit | 96T , 48T , 96T*5 |
