MS Mouse OPG(Osteoprotegerin) ELISA Kit
中文名称:MS-小鼠骨保护素(OPG)酶联免疫吸附测定试剂盒
别称:
TNFRSF11B 、OCIF、TR1
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 125-8000 pg/mL
- 灵敏度: 62.87 pg/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中OPG浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠OPG抗体包被于酶标板上,实验时样品(或标准品)中的小鼠OPG会与包被抗体结合。后依次加入生物素化的抗小鼠OPG抗体和辣根过氧化物酶标记的亲和素,抗小鼠OPG抗体与结合在包被抗体上的小鼠OPG结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,OPG浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中OPG的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 125-8000 pg/mL |
灵敏度 | 62.87 pg/mL |
样本体积 | 25μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠OPG,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
CNC-bZIP protein NFE2L1 regulates osteoclast differentiation in antioxidant-dependent and independent manners
IF: 11.799 -
CD39 Produced from Human GMSCs Regulates the Balance of Osteoclasts and Osteoblasts through the Wnt/β-Catenin Pathway in Osteoporosis
IF: 8.986Journal:MOLECULAR THERAPY -
Enhanced ectopic bone formation by strontium-substituted calcium phosphate ceramics through regulation of osteoclastogenesis and osteoblastogenesis
IF: 7.59 -
Amyloid β peptide promotes bone formation by regulating Wnt/β‐catenin signaling and the OPG/RANKL/RANK system
IF: 4.966Journal:FASEB JOURNAL -
Anti-perimenopausal osteoporosis effects of Erzhi formula via regulation of bone resorption through osteoclast differentiation: A network pharmacology-integrated experimental study
IF: 4.36 -
Overexpressed miR-145 inhibits osteoclastogenesis in RANKL-induced bone marrow-derived macrophages and ovariectomized mice by regulation of Smad3
IF: 3.234Journal:LIFE SCIENCES -
CR6-interacting factor-1 contributes to osteoclastogenesis by inducing receptor activator of nuclear factor κB ligand after radiation
IF: 3.231Journal:World Journal of Stem Cells
E-EL-M0081 | Mouse OPG(Osteoprotegerin) ELISA Kit | 96T , 48T , 96T*5 |