PE Anti-Human IL-4 Antibody[MP4-25D2]

货号：E-AB-F1203D
说明书

说明书

Price: ¥2280.00 ¥900.00 ¥3420.00

Size:

100Tests 20Tests 100Tests×2

应用性： ICFCM
亚型： Rat IgG1, κ
宿主： Rat

Background	IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.
Alternate Names	B-cell IgG differentiation factor;B-cell growth factor 1;BSF-1;IGG1 induction factor;IL-4;Interleukin-4
Swissprot	P05112
Gene ID	3565
Clone No	MP4-25D2
Host	Rat
Reactivity	Human
Application	ICFCM
Isotype	Rat IgG1, κ
Isotype Control	PE Rat IgG1, κ Isotype Control[HRPN]
Conjugation	PE
Conjugation Information	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
Spectrum
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
Storage	This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Expiration date	12个月
Shipping	冰袋

HEK293T cells transiently transfected with pcDNA3.1 plasmid encoding Human IL-4 gene are stained with PE Anti-Human IL-4 Antibody (filled gray histogram) or PE Rat IgG1, κ Isotype Control (empty black histogram).

E-AB-F12030	AF/LE Purified Anti-Human IL-4 Antibody[MP4-25D2]	500μg , 50μg , 1mg
E-AB-F1203A	Purified Anti-Human IL-4 Antibody[MP4-25D2]	100μg , 25μg
E-AB-F1203C	FITC Anti-Human IL-4 Antibody[MP4-25D2]	100Tests , 20Tests , 100Tests×2
E-AB-F1203E	APC Anti-Human IL-4 Antibody[MP4-25D2]	100Tests , 20Tests , 100Tests×2
E-AB-F1203P	PE/Elab Fluor® 594 Anti-Human IL-4 Antibody[MP4-25D2]	100Tests , 20Tests , 100Tests×2

Q1:组织样本检测胞内指标的注意事项有哪些？

①所有样本管均需与实验组一样进行固定破膜，如blank对照，单标组，fmo-iso；②各组固定破膜时间尽量不要相差太久，如果样本量多，建议分批次操作；③对于肿瘤组织样本，由于检测目的细胞量少，需考虑增加抗体用量，上机时间数至少100万个；④建议增加75um微米孔径滤网进行二次过滤，提高免疫细胞纯度和含量；⑤由于刺激阻断时间较长，需要在刺激阻断时间结束前注意无菌操作。
参考资料：
①血样胞内因子实验注意事项的直播回放：https://www.elabscience.cn/resource-flow_cytometry-detail-965。
②肿瘤组织单细胞悬液的制备方案：https://www.elabscience.cn/resource-flow_cytometry-detail-261。

Q2:流式抗体样本什么时候用无菌的耗材？

后续需要进行细胞培养操作的实验都需要使用无菌耗材，如胞内因子检测或流式分选实验。

Q3:人外周血检测胞内因子的注意事项有哪些？

①选择肝素抗凝管；②样本制备：a.人外周血样本：提前稀释红细胞裂解液，现配现用；b.PBMC样本：将样本及试剂复温到18~20℃以后再进行实验；实验环境温度控制在18~20℃；收集的新鲜人血尽量在1 h内进行PBMC分离; 加入血液时，避免冲散分层液面；加完后不要混匀或晃动管。③采血过程中，血样在肝素抗凝管中充分摇匀，避免血凝（肝素抗凝管保存时间一般不要超过12h）；④裂红时间不超过5min，建议做预实验摸索一下最佳时间；⑤无菌操作；⑥刺激和阻断时间建议通过预实验摸索。