Purified Anti-Human CD16 Antibody[3G8]
Price:
Size:
100μg
25μg
- 应用性: FCM;Block
- 亚型: Mouse IgG1, κ
- 宿主: Mouse
Background | CD16 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD16 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD16 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD16 appears to be expressed later in the immune response than CD86. CD16 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. |
Alternate Names | FcγRIII;Fc gamma receptor;Fc gamma receptor 3; |
Swissprot | |
Gene ID | |
Clone No | |
Host | Mouse |
Reactivity | Human |
Application | |
Isotype | Mouse IgG1, κ |
Isotype Control | |
Conjugation | Unconjugated |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide. |
Storage | This product can be stored at 2-8°C for 12 months. Do not freeze. |
Expiration date | 12个月 |
Shipping |
冰袋 |
E-AB-F1236C | FITC Anti-Human CD16 Antibody[3G8] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1236D | PE Anti-Human CD16 Antibody[3G8] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1236E | APC Anti-Human CD16 Antibody[3G8] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1236F | PerCP Anti-Human CD16 Antibody[3G8] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1236H | PE/Cyanine7 Anti-Human CD16 Antibody[3G8] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1236I | PE/Cyanine5.5 Anti-Human CD16 Antibody[3G8] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1236J | PerCP/Cyanine5.5 Anti-Human CD16 Antibody[3G8] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1236L | Elab Fluor® 488 Anti-Human CD16 Antibody[3G8] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1236M | Elab Fluor® 647 Anti-Human CD16 Antibody[3G8] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1236P | PE/Elab Fluor® 594 Anti-Human CD16 Antibody[3G8] | 100Tests , 20Tests , 100Tests×2 |
E-AB-F1236S | Elab Fluor® Red 780 Anti-Human CD16 Antibody[3G8] | 100Tests , 20Tests , 100Tests×2 |
Q1:How to use the blocking agent?
0.5-1 μg (1 μl-2 μL) of blocking antibody was added to 100 μL of cell suspension (containing 1x10^6 cells) and incubated at room temperature for 10 minutes. For Fc receptor-rich cells such as macrophages, the recommended dosage can be up to 2μL.