QuicKey Pro Rat CORT(Corticosterone) ELISA Kit
中文名称:QuicKey Pro-大鼠皮质酮(CORT)酶联免疫吸附测定试剂盒
别称:
Corticosterone
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 4.69-300 ng/mL
- 灵敏度: 1.87 ng/mL
系列介绍 | 与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测大鼠血清、血浆、唾液、尿液中Cort浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用大鼠Cort抗原包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗Cort单克隆抗体,样本(或标准品中)的Cort与包被抗原Cort竞争结合HRP标记的抗Cort单抗上的结合位点,形成包被抗原Cort-HRP标记的抗Cort单抗复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Cort浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中Cort的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 1.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 4.69-300 ng/mL |
灵敏度 | 1.87 ng/mL |
样本体积 | 50μL |
样本类型 | 血清、血浆、唾液、尿液 |
特异性 | 可检测样本中的大鼠Cort,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
数据处理 |
-
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