Rat ANP(Atrial Natriuretic Peptide) ELISA Kit
中文名称:大鼠心钠肽(ANP)酶联免疫吸附测定试剂盒
别称:
NPPA、ANF、ANP、ATFB6、ATRST2、CDD、CDD-ANF、CDP、PND、Atrial natriuretic peptide、Natriuretic peptide A
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用大鼠ANP抗原包被于酶标板上,实验时样品或标准品中的大鼠ANP与包被的大鼠ANP竞争生物素标记的抗大鼠ANP单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,大鼠ANP浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中大鼠ANP的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 50μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠ANP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Water-soluble alkaloids extracted from Aconiti Radix lateralis praeparata protect against chronic heart failure in rats via a calcium signaling pathway
IF: 6.53 -
Natriuretic Peptides—New Targets for Neurocontrol of Blood Pressure via Baroreflex Afferent Pathway
IF: 6.208Journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES -
ANP promotes proliferation and inhibits apoptosis of ovarian granulosa cells by NPRA/PGRMC1/EGFR complex and improves ovary functions of PCOS rats
IF: 5.965Journal:Cell Death & Disease -
Autophagy decreases alveolar epithelial cell injury by regulating the release of inflammatory mediators
IF: 5.546 -
Chlorogenic acid attenuates glucotoxicity in H9c2 cells via inhibition of glycation and PKC α upregulation and safeguarding innate antioxidant status
IF: 3.457Journal:BIOMEDICINE & PHARMACOTHERAPY -
Glucotoxicity results in apoptosis in H9c2 cells via alteration in redox homeostasis linked mitochondrial dynamics and polyol pathway and possible reversal with cinnamic acid
IF: 3.105 -
Synergistic effect of Aconiti Lateralis Radix Praeparata water-soluble alkaloids and Ginseng Radix et Rhizoma total ginsenosides compatibility on acute heart failure rats
IF: 2.813Journal:JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCESPMID:31877430 -
High glucose induced calcium overload via impairment of SERCA/PLN pathway and mitochondrial dysfunction leads to oxidative stress in H9c2 cells and amelioration with ferulic acid
IF: 2.229 -
Differential effects of speed and volume on transfusion-associated circulatory overload: A randomized study in rats
IF: 2.144 -
Exendin‑4 inhibits atrial arrhythmogenesis in a model of myocardial infarction‑induced heart failure via the GLP‑1 receptor signaling pathway
IF: 1.785Journal:Experimental and Therapeutic Medicine