Rat BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
中文名称:大鼠脑源性神经营养因子(BDNF)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠BDNF抗体包被于酶标板上,实验时样品(或标准品)中的大鼠BDNF会与包被抗体结合。后依次加入生物素化的抗大鼠BDNF抗体和辣根过氧化物酶标记的亲和素,抗大鼠BDNF抗体与结合在包被抗体上的大鼠BDNF结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,BDNF浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中BDNF的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠BDNF,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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PLCβ4 driven by cadmium-exposure during gestation and lactation contributes to cognitive deficits by suppressing PIP2/PLCγ1/CREB/BDNF signaling pathway in male offspring
IF: 12.200 -
Extracts of Sideritis scardica and Clinopodium vulgare Alleviate Cognitive Impairments in Scopolamine-Induced Rat Dementia
IF: 5.600Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)PMID:38339117Reactivity:RatSample Type:frontal cortex,hippocampus -
Sildenafil, alone and in combination with imipramine or escitalopram, display antidepressant-like effects in an adrenocorticotropic hormone-induced (ACTH) rodent model of treatment-resistant depression
IF: 5.000Journal: EUROPEAN JOURNAL OF PHARMACOLOGY (2024)PMID:38458412Reactivity:RatSample Type:hippocampal tissue -
Resveratrol-Loaded Pluronic Micelles Ameliorate Scopolamine-Induced Cognitive Dysfunction Targeting Acetylcholinesterase Activity and Programmed Cell Death
IF: 4.900Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity:RatSample Type:cortical tissue,hippocampal tissue -
Development of chitosan lipid nanoparticles to alleviate the pharmacological activity of piperine in the management of cognitive deficit in diabetic rats
IF: 3.800Journal: Scientific Reports (2024)Reactivity:RatSample Type:hippocampi tissue -
Neuroprotective Effect of Marrubium vulgare Extract in Scopolamine-Induced Cognitive Impairment in Rats: Behavioral and Biochemical Approaches
IF: 3.600Journal: Biology-Basel (2024)Reactivity:RatSample Type:frontal cortex,hippocampus tissue -
Neuroprotective effects of saxagliptin against radiation-induced cognitive impairment: Insights on Akt/CREB/SIRT1/BDNF signaling pathway
IF: 3.300Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY (2024)Reactivity:RatSample Type:hippocampal tissue -
The impact of magnesium biotinate and arginine silicate complexes on metabolic dysfunctions, antioxidant activity, inflammation, and neuromodulation in high-fat diet-fed rats
IF: 3.200Journal: CLINICAL AND EXPERIMENTAL MEDICINE (2024)Reactivity:RatSample Type:Serum -
Dynamic changes in the hippocampal memory index and biochemical indices in Sprague Dawley rats exposed to intrauterine kola nut
IF: 3.200Journal: METABOLIC BRAIN DISEASE (2024)Reactivity:RatSample Type:hippocampal tissue -
Beneficial effects of dietary omega 3 polyunsaturated fatty acids on offspring brain development in gestational diabetes mellitus
IF: 2.900Journal: PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS (2024)Reactivity:RatSample Type:brain tissue