Rat CRP(C-Reactive Protein) ELISA Kit
中文名称:大鼠C反应蛋白(CRP)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CRP抗体包被于酶标板上,实验时样品(或标准品)中的大鼠CRP会与包被抗体结合。后依次加入生物素化的抗大鼠CRP抗体和辣根过氧化物酶标记的亲和素,抗大鼠CRP抗体与结合在包被抗体上的大鼠CRP结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,CRP浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中CRP的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠CRP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Investigating the Effects of Gossypetin on Cardiovascular Function in Diet-Induced Pre-Diabetic Male Sprague Dawley Rats
IF: 4.900Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity:RatSample Type:plasma -
Benefits of Puerarin on Metabolic Syndrome and Its Associated Cardiovascular Diseases in Rats Fed a High-Fat/High-Sucrose Diet
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Rutin Promotes Wound Healing by Inhibiting Oxidative Stress and Inflammation in Metformin-Controlled Diabetes in Rats
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Subchronic doses of artemether-lumefantrine, ciprofloxacin and diclofenac precipitated inflammatory and immunological dysfunctions in female Wistar rats
IF: 3.800 -
Antidiabetic and hepatoprotection effect of butterfly pea flower (Clitoria ternatea L.) through antioxidant, anti-inflammatory, lower LDH, ACP, AST, and ALT on diabetes mellitus and dyslipidemia rat
IF: 3.400 -
The effects of resistance training on cardiovascular factors and anti-inflammation in diabetic rats
IF: 3.400 -
Orange Peel ethanolic extract and physical exercise prevent testicular toxicity in streptozocin and high fat diet-induced type 2 diabetes rats via Nrf2/NF-kB signaling: In silico and In vivo studies.
IF: 3.400Journal: Heliyon (2024)Reactivity:RatSample Type:Testicular tissue -
Fermented Rooibos tea (Aspalathus linearis) Ameliorates Sodium Fluoride-Induced Cardiorenal Toxicity, Oxidative Stress, and Inflammation via Modulation of NF-κB/IκB/IκKB Signaling Pathway in Wistar Rats
IF: 3.200Journal: Cardiovascular Toxicology (2024)PMID:38315346Reactivity:RatSample Type:Serum,Kidney,Heart -
Naringin from sweet orange peel improves testicular function in high fat diet-induced diabetic rats by modulating xanthine oxidase/uric acid signaling and maintaining redox balance
IF: 2.900 -
Involvement of pentraxin-3 in the development of hypertension but not left ventricular hypertrophy in male spontaneously hypertensive rats
IF: 2.200