Rat CRP(C-Reactive Protein) ELISA Kit
中文名称:大鼠C反应蛋白(CRP)酶联免疫吸附测定试剂盒
别称:
CRP、PTX1、C-reactive protein、Pentraxin-related、C-Reactive Protein
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CRP抗体包被于酶标板上,实验时样品(或标准品)中的大鼠CRP会与包被抗体结合。后依次加入生物素化的抗大鼠CRP抗体和辣根过氧化物酶标记的亲和素,抗大鼠CRP抗体与结合在包被抗体上的大鼠CRP结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,CRP浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中CRP的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠CRP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Gastroprotective Effect of Azilsartan Through Ameliorating Oxidative Stress, Inflammation, and Restoring Hydroxyproline, and Gastrin Levels in Ethanol-Induced Gastric Ulcer
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Anti-Inflammatory Potentials of the n-Hexane Fraction of Alstonia boonei Stem Bark in Lipopolysaccharide-Induced Inflammation in Wistar Rats
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Photoperiod Conditions Modulate Serum Oxylipins Levels in Healthy and Obese Rats: Impact of Proanthocyanidins and Gut Microbiota
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The dual gastro- and neuroprotective effects of curcumin loaded chitosan nanoparticles against cold restraint stress in rats
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Effects of High Intakes of Fructose and Galactose, with or without Added Fructooligosaccharides, on Metabolic Factors, Inflammation, and Gut Integrity in a Rat Model
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Validation of traditional claims of anti-arthritic efficacy of trans-Himalayan snow mountain garlic (Allium ampeloprasum L.) extract using adjuvant-induced arthritis rat model: A comparative evaluation with normal garlic (Allium sativum L.) and dexamethas
IF: 5.195 -
Network pharmacology combined with pharmacodynamics revealed the anti-inflammatory mechanism of Tanreqing capsule against acute-exacerbation chronic obstructive pulmonary disease
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