Rat HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
中文名称:大鼠低氧诱导因子1α(HIF-1α)酶联免疫吸附测定试剂盒
别称:
HIF1A、HIF-1A、MOP1、PASD8、BHLHe78、Basic helix-loop-helix transcription factor
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠HIF-1α抗体包被于酶标板上,实验时样品(或标准品)中的大鼠HIF-1α会与包被抗体结合。后依次加入生物素化的抗大鼠HIF-1α抗体和辣根过氧化物酶标记的亲和素,抗大鼠HIF-1α抗体与结合在包被抗体上的大鼠HIF-1α结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,HIF-1α浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中HIF-1α的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠HIF-1α,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Effect of Empagliflozin on Thioacetamide-Induced Liver Injury in Rats: Role of AMPK/SIRT-1/HIF-1α Pathway in Halting Liver Fibrosis
IF: 7.675Journal:Antioxidants -
Linagliptin counteracts rotenone’s toxicity in non-diabetic rat model of Parkinson’s disease: Insights into the neuroprotective roles of DJ-1, SIRT-1/Nrf-2 and implications of HIF1-α
IF: 5.195Journal:EUROPEAN JOURNAL OF PHARMACOLOGY -
Investigation on the Q-markers of Bushen Huoxue Prescriptions for DR treatment based on chemometric methods and spectrum-effect relationship
IF: 4.36 -
Anti-Arthritic Effect of Edaravone Against Complete Freund Adjuvant Induced Arthritis via Osteoclast Differentiation and HIF-1α–VEGF–ANG-1 Axis
IF: 4.319Journal:Drug Design Development and Therapy -
Exosomes Derived From Mesenchymal Stem Cells Pretreated With Ischemic Rat Heart Extracts Promote Angiogenesis via the Delivery of DMBT1:
IF: 4.064Journal:CELL TRANSPLANTATION -
Alleviation of remote lung injury following liver ischemia/reperfusion: Possible protective role of vildagliptin
IF: 3.943 -
Twelve weeks of treadmill exercise training with green tea extract reduces myocardial oxidative stress and alleviates cardiomyocyte apoptosis in aging rat: The emerging role of BNIP3 and HIF-1α/IGFBP3 pathway
IF: 3.654 -
Sulforaphane Induces Glioprotection After LPS Challenge
IF: 3.606 -
Metformin ameliorates bladder dysfunction in a rat model of partial bladder outlet obstruction
IF: 3.377Journal:AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGYPMID:33645317 -
Reparative effects of chronic intermittent hypobaric hypoxia pre‑treatment on intervertebral disc degeneration in rats
IF: 2.952