Rat IL-2(Interleukin 2) ELISA Kit
中文名称:大鼠白介素2(IL-2)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-2抗体包被于酶标板上,实验时样品(或标准品)中的大鼠IL-2会与包被抗体结合。后依次加入生物素化的抗大鼠IL-2抗体和辣根过氧化物酶标记的亲和素,抗大鼠IL-2抗体与结合在包被抗体上的大鼠IL-2结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-2浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-2的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠IL-2,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Cang-ai volatile oil alleviates nasal inflammation via Th1/Th2 cell imbalance regulation in a rat model of ovalbumin-induced allergic rhinitis
IF: 4.400Journal: Frontiers in Pharmacology (2024)Reactivity:RatSample Type:Serum -
Mitigation of hypobaric hypoxia induced renal inflammatory alterations by Quercetin prophylaxis
IF: 3.300Journal: Journal of Traditional and Complementary Medicine (2024)Reactivity:RatSample Type:kidney tissue -
Mechanistic analyses reveal that Pueraria montana var. lobata (Willd.) is effective in inhibiting ovarian cancer progression
IF: 3.100Journal: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)Reactivity:RatSample Type:serum -
A study of the effect of natural brown cotton gauze on the healing of infected wounds
IF: 2.000Journal: SKIN RESEARCH AND TECHNOLOGY (2024)Reactivity:RatSample Type:plasma,skin traumatic tissue -
Chenopodium murale Juice Shows Anti-Fungal Efficacy in Experimental Oral Candidiasis in Immunosuppressed Rats in Relation to Its Chemical Profile
IF: 4.600 -
Wound Healing Activity of the Flavonoid-Enriched Fraction of Selaginella bryopteris Linn. against Streptozocin-Induced Diabetes in Rats
IF: 2.600 -
In vivo Combined Vitamin-D and Bone Marrow Mesenchymal Stem Cell (BM-MSCs) Pretreatment Mitigates Hepatic Ischemia-Reperfusion Induced Hepatocellular Damage in Rats
IF: 0.600Journal: JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY (2023)Reactivity:RatSample Type:hepatic -
UPLC-PDA-MS/MS Profiling and Healing Activity of Polyphenol-Rich Fraction of Alhagi maurorum against Oral Ulcer in Rats
IF: 4.658 -
Extracellular vesicles ?produced?by?bone marrow mesenchymal stem cells overexpressing programmed death-ligand 1 ameliorate dextran sodium sulfate-induced ulcerative colitis in rats by regulating Th17/Treg cell balance through PTEN/PI3K/AKT/mTOR axis
IF: 4.369Journal: JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY (2022)PMID:36044618Reactivity:RatSample Type:blood, colon -
Pharmacological Role of Capparis decidua (Forssk.) Edgew in Preventing Cyclophosphamide-induced Myelosuppression and Modulating Innate and Adaptive Immune Response
IF: 2.623