Rat INS(Insulin) ELISA Kit
中文名称:大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 6.25-400 pg/mL
- 灵敏度: 3.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上,实验时样品(或标准品)中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素,抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,INS浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中INS的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 6.25-400 pg/mL |
灵敏度 | 3.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的大鼠INS,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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4-Phenylbutyric Acid Attenuates Pancreatic Beta-Cell Injury in Rats with Experimental Severe Acute Pancreatitis
IF: 2.376Journal:International Journal of Endocrinology -
Cafeteria diet increased adiposity in comparison to high fat diet in young male rats
IF: 2.353Journal:PeerJ -
Time-Restricted Feeding Improved Vascular Endothelial Function in a High-Fat Diet-Induced Obesity Rat Model
IF: 2.304 -
Protective and Therapeutic Effects of Orlistat on Metabolic Syndrome and Oxidative Stress in High-Fat Diet-Induced Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) in Rats: Role on Nrf2 Activation
IF: 2.304Journal:Veterinary Sciences -
Perivascular adipose tissue-derived leptin promotes vascular smooth muscle cell phenotypic switching via p38 mitogen-activated protein kinase in metabolic syndrome rats:
IF: 2.226Journal:EXPERIMENTAL BIOLOGY AND MEDICINE -
The pectin–insulin patch application prevents the onset of peripheral neuropathy-like symptoms in streptozotocin-induced diabetic rats
IF: 2.21Journal:CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY -
The adverse effects of chronic low-dose exposure to nonylphenol on type 2 diabetes mellitus in high sucrose-high fat diet-treated rats
IF: 2.2Journal:Islets -
Edible Bird’s Nest Prevents High Fat Diet-Induced Insulin Resistance in Rats
IF: 2.164Journal:Journal of Diabetes Research -
Evaluating the effects of Juglans regia L. extract on hyperglycaemia and insulin sensitivity in experimental type 2 diabetes in rat
IF: 2.11Journal:ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY -
Effect of neonatal orally administered S-allyl cysteine in high-fructose diet fed Wistar rats
IF: 2.07Journal:Journal of Developmental Origins of Health and Disease