Rat MMP-2(Matrix Metalloproteinase 2) ELISA Kit
中文名称:大鼠基质金属蛋白酶2(MMP-2)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 0.31-20 ng/mL
- 灵敏度: 0.19 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠MMP-2抗体包被于酶标板上,实验时样品(或标准品)中的大鼠MMP-2会与包被抗体结合。后依次加入生物素化的抗大鼠MMP-2抗体和辣根过氧化物酶标记的亲和素,抗大鼠MMP-2抗体与结合在包被抗体上的大鼠MMP-2结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,MMP-2浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中MMP-2的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.31-20 ng/mL |
灵敏度 | 0.19 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠MMP-2,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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The therapeutic potential of 1, 25-dihydroxy vitamin D3 on cisplatin-affected neurological functions is associated with the regulation of oxidative stress and inflammatory markers as well as levels of MMP2/9
IF: 3.200Journal: METABOLIC BRAIN DISEASE (2024)Reactivity:RatSample Type:serum -
Dynamic expression of H19 and MALAT1 and their correlation with tumor progression biomarkers in a multistage hepatocarcinogenesis model
IF: 3.600Journal: CELL BIOCHEMISTRY AND FUNCTION (2023)DOI:10.1002/cbf.3785PMID:36861261Reactivity:RatSample Type:liver tumors -
Patuletin from Tagetes patula: an Inhibitor of MMP-2 and MMP-9 in Collagen-Induced Arthritis Rat Model and Virtual Screening Analysis
IF: 1.600Journal: Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2023)Reactivity:RatSample Type:plasma -
Protective Effect of Liposomal Epigallocatechin-Gallate in Experimental Gentamicin-Induced Hepatotoxicity
IF: 7.675 -
Alleviation of liver cirrhosis and associated portal-hypertension by Astragalus species in relation to their UPLC-MS/MS metabolic profiles: a mechanistic study
IF: 4.996 -
Boswellia serrata Extract, 5-Loxin?, Prevents Joint Pain and Cartilage Degeneration in a Rat Model of Osteoarthritis through Inhibition of Inflammatory Responses and Restoration of Matrix Homeostasis
IF: 2.650Journal: Evidence-based Complementary and Alternative Medicine (2022)Reactivity:RatSample Type:serum -
Does α-lipoic acid therapeutically effective against experimentally induced-acute pulpitis in rats?
IF: 1.719 -
Water-soluble alkaloids extracted from Aconiti Radix lateralis praeparata protect against chronic heart failure in rats via a calcium signaling pathway
IF: 6.530 -
Design, in vitro bioactivity and in vivo influence on oxidative stress and matrix metalloproteinases of bioglasses in experimental skin wound
IF: 3.849Journal: JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021)PMID:34438314Reactivity:RatSample Type:skin -
Anti-Inflammatory Effects of Melatonin and 5-Methoxytryptophol on Lipopolysaccharide-Induced Acute Pulpitis in Rats
IF: 3.411Journal: Biomed Research International (2021)PMID:33628825Reactivity:RatSample Type:Serum,Tissue homogenate(pulp)
Q1:ELISA可以测细胞上清液MMP-2和MMP-9吗?我看其他人写的论文,除了测这两个指标表达量,还要测活性,ELISA是不是可以测活性?跟明胶酶谱法有什么区别?
这两款试剂盒可以用于您的细胞上清的检测的。不过ELISA检测细胞上清受到培养基组分、细胞生长情况、以及外部药物刺激条件等因素的影响,建议您正式实验前先开展预实验。
明胶酶谱法是利用SDS与样品中的MMPs的可逆性结合,将样本中的MMP-2和MMP-9分离,再通过二价金属离子缓冲体系恢复两者的活性,该试验方法是专门针对MMP-2和MMP-9的活性检测的。而ELISA是通过抗原抗体的结合反应去检测待测物的含量(即浓度),即通过MMP-2和MMP-9的特异性抗体去捕获样本的MMP-2和MMP-9。两者的实验原理不同,ELISA无法检测MMP-2和MMP-9的活性。