Rat MMP-3(Matrix Metalloproteinase 3) ELISA Kit
中文名称:大鼠基质金属蛋白酶3(MMP-3)酶联免疫吸附测定试剂盒
别称:
MMP3、CHDS6、SL-1、STMY、STMY1、STR1、Progelatinase
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠MMP-3抗体包被于酶标板上,实验时样品(或标准品)中的大鼠MMP-3会与包被抗体结合。后依次加入生物素化的抗大鼠MMP-3抗体和辣根过氧化物酶标记的亲和素,抗大鼠MMP-3抗体与结合在包被抗体上的大鼠MMP-3结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,MMP-3浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中MMP-3的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | 比色法 |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠MMP-3,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Huangbai liniment and berberine promoted wound healing in high-fat diet/Streptozotocin-induced diabetic rats
IF: 6.53 -
PARP-1 inhibition attenuates the inflammatory response in the cartilage of a rat model of osteoarthritis
IF: 5.853 -
Protective effect of ethanolic extract of Echinacea purpurea contained nanoparticles on meniscal/ligamentous injury induced osteoarthritis in obese male rats
IF: 4.38 -
Role of Glutamine-Glutamate/GABA cycle and potential target GLUD2 in alleviation of rheumatoid arthritis by Tripterygium hypoglaucum (levl.) Hutch based on metabolomics and molecular pharmacology
IF: 4.36 -
Vernonia amygdalina inhibited osteoarthritis development by anti‐inflammatory and anticollagenase pathways in cartilage explant and osteoarthritis‐induced rat model
IF: 3.766Journal:PHYTOTHERAPY RESEARCHDOI:10.1002/ptr.6366 -
Inhibition effect of Caragana sinica root extracts on Osteoarthritis through MAPKs, NF-κB signaling pathway
IF: 3.738 -
Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model
IF: 3.411 -
Effects of alpha‐lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro‐CT analysis
IF: 3.331Journal:INTERNATIONAL ENDODONTIC JOURNAL -
prevented osteoarthritis cartilage degeneration by attenuating joint inflammation and collagen breakdown in postmenopausal rat model
IF: 3.304Journal:INFLAMMOPHARMACOLOGY -
Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study
IF: 3.092Journal:PHYTOTHERAPY RESEARCHDOI:10.1002/ptr.5949