Rat NFE2L2(Nuclear Factor, Erythroid Derived 2, Like 2) ELISA Kit
中文名称:大鼠红细胞衍生核因子2样蛋白2(NFE2L2)酶联免疫吸附测定试剂盒
别称:
Nrf2
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠NFE2L2抗体包被于酶标板上,实验时样品(或标准品)中的大鼠NFE2L2会与包被抗体结合。后依次加入生物素化的抗大鼠NFE2L2抗体和辣根过氧化物酶标记的亲和素,抗大鼠NFE2L2抗体与结合在包被抗体上的大鼠NFE2L2结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,NFE2L2浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中NFE2L2的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠NFE2L2,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
The dual gastro- and neuroprotective effects of curcumin loaded chitosan nanoparticles against cold restraint stress in rats
IF: 6.53 -
Acetate circumvents impaired metabolic switch in skeletal muscle of letrozole-induced PCOS rat model by suppression of PDK4/NLRP3
IF: 4.893Journal:NUTRITION -
Dimethyl fumarate attenuates 2-VO-induced vascular dementia via activating the Nrf2 signaling pathway in rats
IF: 4.473 -
Activation of the Nrf2/HO-1 signaling pathway by dimethyl fumarate ameliorates complete Freund's adjuvant-induced arthritis in rats
IF: 4.432 -
Plantain-based diet decreases oxidative stress and inflammatory markers in the testes of rats exposed to atrazine
IF: 3.842Journal:MOLECULAR AND CELLULAR BIOCHEMISTRY -
Short chain fatty acid, acetate restores ovarian function in experimentally induced PCOS rat model
IF: 3.752 -
Essential Trace Elements Prevent the Impairment in the Retention Memory, Cerebral Cortex, and Cerebellum Damage in Male Rats Exposed to Quaternary Metal Mixture by Up-regulation, of Heme Oxygynase-1 and Down-regulation of Nuclear Factor Erythroid 2-relate
IF: 3.708 -
Protective Effects of Tyrosol Against DSS-Induced Ulcerative Colitis in Rats
IF: 2.939Journal:INFLAMMATION -
Zn and Se abrogate heavy metal mixture induced ovarian and thyroid oxido-inflammatory effects mediated by activation of NRF2-HMOX-1 in female albino rats
Journal:Current Research in Toxicology -
Acetate supplementation restores testicular function by modulating Nrf2/PPAR-γ in high fat diet-induced obesity in Wistar rats
Journal:Journal of Diabetes and Metabolic Disorders