Rat NFKB-p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
中文名称:大鼠核因子κB亚基p105(NFKB-p105)酶联免疫吸附测定试剂盒
别称:
NFKB1、NF-KB1、NF-Kappa-B、NFKB-p105、NFKB-p50、P105、P50、EBP-1、KBF1
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠NFKB-p105抗体包被于酶标板上,实验时样品(或标准品)中的大鼠NFKB-p105会与包被抗体结合。后依次加入生物素化的抗大鼠NFKB-p105抗体和辣根过氧化物酶标记的亲和素,抗大鼠NFKB-p105抗体与结合在包被抗体上的大鼠NFKB-p105结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,NFKB-p105浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中NFKB-p105的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠NFKB-p105,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Glutamine restores testicular glutathione-dependent antioxidant defense and upregulates NO/cGMP signaling in sleep deprivation-induced reproductive dysfunction in rats
IF: 6.53 -
Banana peel ameliorated hepato-renal damage and exerted anti-inflammatory and anti-apoptotic effects in metal mixture mediated hepatic nephropathy by activation of Nrf2/ Hmox-1 and inhibition of Nfkb pathway
IF: 5.572 -
Protective effects of hesperidin through attenuation of Ki67 expression against DMBA-induced breast cancer in female rats
IF: 5.037 -
Zinc and selenium mitigated heavy metals mixture (Pb, Al, Hg and Mn) mediated hepatic-nephropathy via modulation of oxido-inflammatory status and NF‑kB signaling in female albino rats
IF: 4.571 -
Dimethyl fumarate attenuates 2-VO-induced vascular dementia via activating the Nrf2 signaling pathway in rats
IF: 4.473 -
Menhaden fish oil attenuates postpartum depression in rat model via inhibition of NLRP3-inflammasome driven inflammatory pathway
IF: 4.221Journal:Journal of Traditional and Complementary Medicine -
Effect of lobeglitazone on motor function in rat model of Parkinson’s disease with diabetes co-morbidity
IF: 4.079Journal:BRAIN RESEARCH BULLETIN -
Tetramethylpyrazine Attenuates Cognitive Impairment Via Suppressing Oxidative Stress, Neuroinflammation, and Apoptosis in Type 2 Diabetic Rats
IF: 3.996 -
Acetate restores hypothalamic-adipose kisspeptin status in a rat model of PCOS by suppression of NLRP3 immunoreactivity
IF: 3.925 -
Essential Trace Elements Prevent the Impairment in the Retention Memory, Cerebral Cortex, and Cerebellum Damage in Male Rats Exposed to Quaternary Metal Mixture by Up-regulation, of Heme Oxygynase-1 and Down-regulation of Nuclear Factor Erythroid 2-relate
IF: 3.708