Rat PCSK9(Proprotein convertase subtilisin/kexin type 9) ELISA Kit
中文名称：大鼠前蛋白转化酶枯草溶菌素9(PCSK9)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.47 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠PCSK9抗体包被于酶标板上，实验时样品（或标准品）中的大鼠PCSK9会与包被抗体结合。后依次加入生物素化的抗大鼠PCSK9抗体和辣根过氧化物酶标记的亲和素，抗大鼠PCSK9抗体与结合在包被抗体上的大鼠PCSK9结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，PCSK9浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中PCSK9的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.47 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠PCSK9，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Organosulfur Compounds, S-Allyl-L-Cysteine and S-Ethyl-L-Cysteine, Target PCSK-9/LDL-R-Axis to Ameliorate Cardiovascular, Hepatic, and Metabolic Changes in High Carbohydrate and High Fat Diet-Induced Metabolic Syndrome in Rats
IF: 6.100 -
High-salt diet induces dyslipidemia through the SREBP2/PCSK9 pathway in dahl salt-sensitive rats
IF: 3.900 -
Low-dose spironolactone combats dyslipidemia and hepatic inflammation by modulating PCSK9 in rat model of polycystic ovarian syndrome
IF: 3.800Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2023)PMID：37328115Reactivity：RatSample Type：Plasma,liver -
PCSK9 promotes arterial medial calcification
IF: 6.847 -
Suppression of PCSK9/NF-kB-dependent pathways by acetate ameliorates cardiac inflammation in a rat model of polycystic ovarian syndrome
IF: 6.780 -
Curcumin nicotinate decreases serum LDL cholesterol through LDL receptor-mediated mechanism
IF: 5.195 -
Rescue effect of sodium acetate in diabetes mellitus-associated testicular dysfunction is accompanied by PCSK9 modulation
IF: 4.079 -
Effect of Sleeve Gastrectomy on Proprotein Convertase Subtilisin/Kexin Type 9 (Pcsk9) Content and Lipid Metabolism in the Blood Plasma and Liver of Obese Wistar Rats
IF: 4.171
实验操作视频
