Rat VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit
中文名称:大鼠血管内皮细胞生长因子A(VEGF-A)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠VEGF-A抗体包被于酶标板上,实验时样品(或标准品)中的大鼠VEGF-A会与包被抗体结合。后依次加入生物素化的抗大鼠VEGF-A抗体和辣根过氧化物酶标记的亲和素,抗大鼠VEGF-A抗体与结合在包被抗体上的大鼠VEGF-A结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,VEGF-A浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中VEGF-A的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠VEGF-A,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Curative effect and mechanisms of Radix Arnebiae oil on burn wound healing in rats
IF: 3.007Journal:PLANTA MEDICA -
Protective effects of essential oil from Fructus Alpiniae zerumbet on retinal Müller gliosis via the PPAR-γ-p-CREB signaling pathway
IF: 2.96 -
Impact of repeated intravenous infusions of umbilical cord–derived versus bone marrow–derived mesenchymal stem cells on angiogenesis in a pregnant experimentally induced deep venous thrombosis rat model
IF: 2.723Journal:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL -
The Effect of Waterpipe Tobacco Smoking on Bone Healing Following Femoral Fractures in Male Rats
IF: 2.718Journal:Frontiers in Surgery -
Molecular Mechanism of Traditional Chinese Ointment of Xuzhou Qufu Shengji in Infected Wounds
IF: 2.63Journal:Evidence-based Complementary and Alternative MedicinePMID:35069758 -
Potential stem cell—Conditioned medium and their derived exosomes versus omeprazole in treatment of experimental model of gastric ulcer
IF: 2.479Journal:ACTA HISTOCHEMICA -
Combination of mild therapeutic hypothermia and adipose-derived stem cells for ischemic brain injury
IF: 2.234Journal:Neural Regeneration Research -
Electroacupuncture pretreatment promotes angiogenesis via hypoxia-inducible factor 1α and vascular endothelial growth factor in a rat model of chronic myocardial ischemia:
IF: 2.129Journal:Acupuncture in Medicine -
Sildenafil ameliorates Alzheimer disease via the modulation of vascular endothelial growth factor and vascular cell adhesion molecule-1 in rats:
IF: 2.067Journal:HUMAN & EXPERIMENTAL TOXICOLOGY -
PTEN/PI3K/VEGF signaling pathway involved in the protective effect of xanthine oxidase inhibitor febuxostat against endometrial hyperplasia in rats:
IF: 2.067Journal:HUMAN & EXPERIMENTAL TOXICOLOGY