Recombinant E.coli Tryptophan Synthase β Chain/Trp B Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P0A879
| 别称 |
Tryptophan synthase beta chain;trpB
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| 表达系统 |
E.coli
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| 序列 |
Thr2-Ile397
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| 蛋白编码 |
P0A879
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| 种属 |
E.coli
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| 计算分子量 |
43.8 kDa
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| 表观分子量 |
38-48 kDa
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| 标签 |
N-His
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
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| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Sucrose, 0.05% Tween 80, pH 8.5.
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| 复溶方法 |
Not Applicable
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| 背景 |
Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from animals such as humans. Tryptophan synthase typically exists as an α-ββ-α complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation.
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实验操作视频
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