Recombinant Human 4-1BB/TNFRSF9 Protein (Fc & His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q07011
- 应用性： ELISA
| 别称 |
CD137;ILA;TNFRSF9;4-1BB ligand receptor;CDw137;T-cell antigen 4-1BB homolog;T-cell antigen ILA
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| 表达系统 |
HEK293 Cells
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| 序列 |
Leu24-Gln186
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| 蛋白编码 |
Q07011
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| 种属 |
Human
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| 计算分子量 |
44.0 kDa
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| 表观分子量 |
55-75 kDa
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| 标签 |
C-Fc-His
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| 生物活性 |
Immobilized Human 4-1BB-Fc-His at 2μg/ml (100 μl/well)can bind Anti-Human CD137 mAb .The ED50 of Anti-Human CD137 mAb is 0.41ug/ml.
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Tumor necrosis factor receptor superfamily member 9(TNFRSF9) is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is a single-pass type I membrane protein which contains 4 TNFR-Cys repeats. The human and mouse proteins share 60% amino acid sequence identity. It is absent from naive T cells; but upregulated and continually expressed following T cell activation. It is a receptor for TNFSF9/4-1BBL; and possibly active during T cell activation.
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实验操作视频
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