Recombinant Human Apolipoprotein A-I/ApoAI Protein (His Tag, E. coli)
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- 表达系统： E.coli
- 蛋白编码： P02647
| 别称 |
Apolipoprotein A-I;Apo-AI;ApoA-I;Apolipoprotein A1;APOA1
|
| 表达系统 |
E.coli
|
| 序列 |
Arg19-Gln267
|
| 蛋白编码 |
P02647
|
| 种属 |
Human
|
| 计算分子量 |
30.2 kDa
|
| 表观分子量 |
28 kDa
|
| 标签 |
C-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 6% Sucrose, 4% Mannitol, 50mM Nacl, 0.05% Tween 80, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Apolipoprotein A1 (APOA1) is a secreted protein which belongs to the Apolipoprotein A1/A4/E family. APOA1 is the major protein component of high density lipoprotein (HDL) in plasma. APOA1 plays a critical role in various biological processes; such as Cholesterol metabolism; Lipid metabolism and transport; Steroid metabolism. APOA1 promotes cholesterol efflux from tissues to the liver and thus helps to clear cholesterol from arteries. Defects in this gene resulted in HDL deficiencies; including Tangier disease (TGD); systemic non-neuropathic amyloidosis; premature coronary artery disease; hepatosplenomegaly and progressive muscle wasting and weakness. In addition; ApoA-I is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin.
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实验操作视频
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