Recombinant Human ASS1 Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P00966
| 别称 |
Argininosuccinate Synthase;Citrulline--Aspartate Ligase;ASS1;ASS
|
| 表达系统 |
E.coli
|
| 序列 |
Met 1-Lys412
|
| 蛋白编码 |
P00966
|
| 种属 |
Human
|
| 计算分子量 |
42.8 kDa
|
| 表观分子量 |
50 kDa
|
| 标签 |
N-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 50mM Imidazole, 1mM DTT, 40% Glycerol, pH 7.5.
|
| 复溶方法 |
Not Applicable
|
| 背景 |
Argininosuccinate Synthase (ASS1) is an urea cycle enzyme with a tetrameric structure composed of identical subunits. ASS1 is involved in the synthesis of arginine and catalyzes that condensation of citrulline and aspartate to argininosuccinate using ATP. ASS1 is important to the urea cycle as it catalyzes the important second last step in the arginine biosynthetic pathway. ASS1 mainly expressed in periportal hepatocytes, but also in most other body tissues. A deficiency of ASS1 causes citrullinemia (CTLN1), an autosomal recessive disease which is characterized by severe vomiting spells and mental retardation.
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实验操作视频
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