Recombinant Human ATG5 Protein
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- 表达系统： E.coli
- 蛋白编码： Q9H1Y0
| 别称 |
Autophagy protein 5;APG5-like;Apoptosis-specific protein;APG5L;ASP;ATG5;
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| 表达系统 |
E.coli
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| 序列 |
Met 1-Asp275
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| 蛋白编码 |
Q9H1Y0
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| 种属 |
Human
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| 计算分子量 |
32.4 kDa
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| 表观分子量 |
33 kDa
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| 标签 |
None
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| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
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| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 0.2M NaCl, 1mM DTT, 40% Glycerol, pH 8.0.
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| 复溶方法 |
Not Applicable
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| 背景 |
ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.
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实验操作视频
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